Thrombosis-related diseases represent the leading causes of disability or death worldwide. However, conventional thrombolytic therapies are subjected to narrow therapeutic window, short circulation half-life and bleeding. Herein, we rationally design and develop a safe and efficient nonpharmaceutical thrombolysis strategy based on a specific piezocatalytic effect arising from platelet membrane (PM)-conjugated two-dimensional (2D) piezoelectric selenene, Se-PM nanosheets (NSs). The 2D selenene is fabricated from nonlayered bulk selenium powder by a facile liquid-phase exfoliation method, and the PM conjugation confers selenene with the distinct thrombus-homing feature. Under ultrasonic activation, the piezoelectric characteristic of selenene triggers electrons and holes separation, resulting in generation of reactive oxygen species (ROS) by reacting with surrounding H₂O and O₂ in the thrombosis microenvironment for thrombolysis. Both systematic in vitro and in vivo assessments demonstrate that the biocompatible Se-PM NSs efficiently degrade erythrocytes, fibrin and artificial blood clots under ultrasound irradiation. Compared to the clinical thrombolytic drug urokinase plasminogen activator, the engineered Se-PM NSs possess excellent thrombolytic efficacy by single treatment in the tail thrombosis animal model without bleeding risk. The engineered Se-PM nanoplatform marks an exciting jumping-off point for research into the application of piezocatalysis in clinical treatment of thrombosis.

血栓相关疾病是全世界残疾或死亡的主要原因。然而，传统的溶栓疗法存在治疗窗窄、循环半衰期短和出血等问题。在此，我们基于血小板膜（PM）共轭二维（2D）压电硒烯Se-PM纳米片（NSs）产生的特定压电催化效应，合理设计和开发一种安全有效的非药物溶栓策略。二维硒烯是通过简单的液相剥离方法由非层状散装硒粉制成的，PM 结合赋予硒烯独特的血栓归巢特征。_{在超声激活下，硒烯的压电特性触发电子和空穴分离，与血栓微环境中周围的H 2}O和O₂反应产生活性氧(ROS)，从而实现溶栓。系统的体外和体内均表明，生物相容性 Se-PM NS 在超声波照射下可有效降解红细胞、纤维蛋白和人造血凝块。与临床溶栓药物尿激酶纤溶酶原激活剂相比，工程化Se-PM NSs在尾部血栓动物模型中单次治疗具有优异的溶栓效果，且无出血风险。工程化的 Se-PM 纳米平台标志着压电催化在血栓形成临床治疗中应用研究的一个令人兴奋的起点。