In this study, the dried herb of three Vincetoxicum species was subjected to supercritical carbon dioxide (SC-CO₂) extraction for the recovery and characterisation of lipophilic compounds. The differences in extraction yield were not remarkable for the analyzed species; however, the effect of herb harvesting time at different year was quite significant. Two times higher yields of lipids were obtained from the plants collected in September 2020 compared to those collected in June 2022, e.g., from 1.39 to 3.01%. Linoleic, linolenic, oleic and palmitic acids were the major fatty acids in the extracted oils. β-sitosterol was the major phytosterol (from 4.84 to 10.74 mg/g), followed by stigmasterol, while vitamin E (α-tocopherol) was the dominant tocopherol in Vincetoxicum species extracts.

CO₂ extracts had similar antioxidant capacity and total phenolic content determined by different methods. Isovaleric acid, butyrolactone, hexanoic acid were the major volatile components identified in almost all plants extracts, however based on the integrated peak area significant quantitative variations were observed for different species and harvesting period (year). In conclusion, this study is the first report on lipophilic fraction of Vincetoxicum species recovered by SC-CO₂, which demonstrates the presence of various bioactive components in the studied botanicals.

在本研究中，对三种毒藤属植物的干燥药草进行超临界二氧化碳 (SC-CO ₂ ) 萃取，以回收和表征亲脂性化合物。对于所分析的物种来说，提取率的差异并不显着；但不同年份采收时间的影响相当显着。与 2022 年 6 月收集的植物相比，2020 年 9 月收集的植物获得的脂质产量高出两倍，例如 1.39% 至 3.01%。亚油酸、亚麻酸、油酸和棕榈酸是提取油中的主要脂肪酸。β-谷甾醇是主要的植物甾醇（从 4.84 到 10.74 mg/g），其次是豆甾醇，而维生素 E（α-生育酚）是Vincetoxum物种提取物中的主要生育酚。

不同方法测定CO ₂提取物具有相似的抗氧化能力和总酚含量。异戊酸、丁内酯、己酸是几乎所有植物提取物中鉴定出的主要挥发性成分，然而，根据积分峰面积，不同物种和收获期（年）观察到显着的定量变化。总之，这项研究是第一份关于通过 SC-CO _2回收的毒藤菌亲脂部分的报告，这证明了所研究的植物药中存在各种生物活性成分。