Supercritical fluid extraction using CO₂ (SCFE-CO₂) was used to examine extraction efficiency of bioactive compounds from irregular-shaped Lion's Mane (LM) mushroom by-products. Optimal SCFE-CO₂ conditions (46.38 °C, 100 bar, and 0.99 mL/min of EtOH flow rate) for maximum yield (a mass fraction of 11.64%), ergosterol (3.74 mg/g dried LM), total phenolic content, TPC (815.93 µg GAE/g dried LM), and antioxidant activity (42.97 µg TE/g dried LM) were determined using following parameters: temperature (40 °C to 60 °C), pressure (100 bar to 300 bar), and EtOH flow rate (0 mL/min to 1 mL/min). EtOH flow rate was a key factor in raising extraction yield and quality. SCFE-CO₂ was equally effective compared to conventional extraction (Maceration) for LM, as there were no significant differences (p ≥ 0.05) in yield, ergosterol content, TPC, or antioxidant activity. This green extraction technology could be an excellent alternative method for recovering bioactive compounds from medicinal mushrooms.

_{使用CO 2}超临界流体萃取(SCFE-CO ₂ ) 来检查从不规则形状的狮鬃菇(LM) 蘑菇副产物中提取生物活性化合物的效率。最佳 SCFE-CO ₂条件（46.38 °C、100 bar 和 0.99 mL/min 的 EtOH 流速）可实现最大产量（质量分数 11.64%）、麦角甾醇（3.74 mg/g 干燥的 LM）、总酚含量、使用以下参数测定TPC（815.93 µg GAE/g 干燥 LM）和抗氧化活性（42.97 µg TE/g 干燥 LM）：温度（40 °C 至 60 °C）、压力（100 bar 至 300 bar）和EtOH 流速（0 mL/min 至 1 mL/min）。乙醇流量是提高提取率和质量的关键因素。对于 LM， SCFE-CO ₂与传统提取（浸渍）同样有效，因为在产量、麦角甾醇含量、TPC 或抗氧化活性方面没有显着差异 (p ≥ 0.05)。这种绿色提取技术可能是从药用蘑菇中回收生物活性化合物的绝佳替代方法。