Polyhydroxyalkanoates (PHAs) are biodegradable polyesters produced by a wide range of microorganisms, including extremophiles. These unique microorganisms have gained interest in PHA production due to their ability to utilise low-cost carbon sources under extreme conditions. In this study, Halomonas alkaliantarctica was examined with regards to its potential to produce PHAs using crude glycerol from biodiesel industry as the only carbon source. We found that cell dry mass concentration was not dependent on the applying substrate concentration. Furthermore, our data confirmed that the analysed halophile was capable of metabolising crude glycerol into poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer within 24 h of the cultivation without addition of any precursors. Moreover, crude glycerol concentration affects the repeat units content in the purified PHAs copolymers and their thermal properties. Nevertheless, a differential scanning calorimetric and thermogravimetric analysis showed that the analysed biopolyesters have properties suitable for various applications. Overall, this study described a promising approach for the valorisation of crude glycerol as a future strategy of industrial waste management to produce high value microbial biopolymers.

中文翻译：

---

碱南极盐单胞菌作为从生物柴油衍生的甘油生产聚（3-羟基丁酸酯-co-3-羟基戊酸酯）的平台

聚羟基脂肪酸酯 (PHA) 是由包括极端微生物在内的多种微生物产生的可生物降解聚酯。这些独特的微生物因其在极端条件下利用低成本碳源的能力而引起了人们对 PHA 生产的兴趣。在这项研究中，考察了碱南极盐单胞菌使用生物柴油工业中的粗甘油作为唯一碳源生产 PHA 的潜力。我们发现细胞干质量浓度不依赖于所应用的底物浓度。此外，我们的数据证实，所分析的嗜盐菌能够在培养后 24 小时内将粗甘油代谢为聚（3-羟基丁酸酯-co -3-羟基戊酸酯）共聚物，而无需添加任何前体。此外，粗甘油浓度影响纯化PHAs共聚物中重复单元的含量及其热性能。然而，差示扫描量热和热重分析表明，所分析的生物聚酯具有适合各种应用的性能。总体而言，这项研究描述了一种有前途的粗甘油增值方法，作为工业废物管理的未来战略，以生产高价值的微生物生物聚合物。