Although it is in its early stages, canine induced pluripotent stem cells (ciPSCs) hold great potential for innovative translational research in regenerative medicine, developmental biology, drug screening, and disease modeling. However, almost all ciPSCs were generated from fibroblasts, and available canine cell sources for reprogramming are still limited. Furthermore, no report is available to generate ciPSCs under feeder-free conditions because of their low reprogramming efficiency. Here, we reanalyzed canine pluripotency-associated genes and designed canine LIN28A, NANOG, OCT3/4, SOX2, KLF4, and C-MYC encoding Sendai virus vector, called 159cf. and 162cf. We demonstrated that not only canine fibroblasts but also canine urine-derived cells, which can be isolated using a noninvasive and straightforward method, were successfully reprogrammed with or without feeder cells. ciPSCs existed in undifferentiated states, differentiating into the three germ layers in vitro and in vivo. We successfully generated ciPSCs under feeder-free conditions, which can promote studies in veterinary and consequently human regenerative medicines.

尽管还处于早期阶段，但犬诱导多能干细胞 (ciPSC) 在再生医学、发育生物学、药物筛选和疾病建模领域的创新转化研究中具有巨大潜力。然而，几乎所有 ciPSC 都是由成纤维细胞产生的，可用于重编程的犬细胞来源仍然有限。此外，由于重编程效率低，目前还没有在无饲养层条件下生成 ciPSC 的报告。在这里，我们重新分析了犬多能性相关基因，并设计了编码犬LIN28A、NANOG、OCT3/4、SOX2、KLF4和C-MYC的仙台病毒载体，称为159cf。和162cf。我们证明，不仅可以使用非侵入性且简单的方法分离犬成纤维细胞，而且可以使用或不使用饲养细胞成功地对犬尿液来源的细胞进行重编程。ciPSCs以未分化状态存在，在体外和体内分化成三个胚层。我们在无饲养条件下成功生成了 ciPSC，这可以促进兽医乃至人类再生医学的研究。