The combination of amoxicillin (AMX) and clavulanic acid (CLA) is one of the most widely used antibiotic medicines in the world. This paper describes a novel electrochemical method for the fast sequential determination of AMX and CLA using batch injection analysis with amperometric detection (BIA-AD) and boron-doped diamond (BDD) as the working electrode. In the proposed strategy, AMX was selectively detected at +1.3 V and both compounds (AMX+CLA) at +1.8 V. The oxidation current from CLA was accessed by subtracting the currents detected at both potential pulses (i_{+1.8 V}–i_+1.3V) and using a simple correction factor. The developed method proved to be simple, cost-effective, precise (RSD<1.3 %, n=10), rapid (>200 injection h⁻¹), required simple sample preparation steps (dissolution and dilution only) and can be used in laboratories with minimal infrastructure (portable characteristics). The limits of detection were 0.31 (0.13) and 1.99 (0.47) μmol L⁻¹ (mg L⁻¹) for AMX and CLA, respectively. The results obtained by the proposed BIA-AD method were comparable to those obtained by capillary electrophorese (95 % confidence level).

中文翻译：

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使用批量进样分析和安培检测快速连续测定药物样品中的阿莫西林和克拉维酸盐

阿莫西林（AMX）和克拉维酸（CLA）的组合是世界上使用最广泛的抗生素药物之一。本文描述了一种新型电化学方法，使用电流检测批量注射分析 (BIA-AD) 和硼掺杂金刚石 (BDD) 作为工作电极，快速连续测定 AMX 和 CLA。在所提出的策略中，AMX 在 +1.3 V 下被选择性检测，两种化合物 (AMX+CLA) 在 +1.8 V 下被选择性检测。通过减去在两个电势脉冲 (i _{+1.8 V} –i _{+ 1.3V}）并使用简单的校正因子。所开发的方法被证明是简单、经济高效、精确（RSD <1.3 %，n=10）、快速（> 200 进样 h ^-1）、所需的简单样品制备步骤（仅溶解和稀释）并且可用于基础设施最少的实验室（便携式特性）。AMX 和 CLA的检测限分别为 0.31 (0.13) 和 1.99 (0.47) μmol L ^-1 (mg L ^-1 )。所提出的 BIA-AD 方法获得的结果与毛细管电泳获得的结果相当（95% 置信水平）。