The level of folate receptor (FR) has become one of the independent factors for measuring human tumor diseases. The precise quantification of FR is helpful for the early diagnosis and subsequent treatment of tumors. The modification of electrodes is a key issue in ensuring and enhancing the electrochemical biosensing ability. In this study, we in-situ synthesized a nanocomposite material with excellent conductivity and stability by grafting first-generation poly(amidoamine) dendrimers onto the MXene (Ti₃C₂T_X) as the immobilized matrix (PAMAM@MXene). An electrochemical sensor was developed for FR monitor by loading the PAMAM@MXene on screen-printed carbon electrodes (SPCEs). Scanning electron microscopy (SEM) supported the effective synthesis of PAMAM@MXene. Under optimal conditions, the prepared sensor achieved the quantification of FR with a wide range of concentrations from 10 ng/mL to 1000 ng/mL with a detection limit (LOD) of 5.6 ng/mL. It also exhibited satisfactory selectivity, reproducibility, and stability, which provided the possibility for expanding new pathways in the detection of clinical FR.

叶酸受体（FR）水平已成为衡量人类肿瘤疾病的独立因素之一。FR的精确量化有助于肿瘤的早期诊断和后续治疗。电极的修饰是保证和增强电化学生物传感能力的关键问题。在本研究中，我们通过将第一代聚酰胺胺树枝状大分子接枝到作为固定基质的MXene（Ti ₃ C ₂ T _{X ）上（PAMAM@MXene），原位合成了具有优异导电性和稳定性的纳米复合材料。}通过将 PAMAM@MXene 加载到丝网印刷碳电极 (SPCE) 上，开发了一种用于 FR 监测的电化学传感器。扫描电子显微镜（SEM）支持PAMAM@MXene的有效合成。在最佳条件下，所制备的传感器实现了10 ng/mL至1000 ng/mL范围内的FR定量，检测限(LOD)为5.6 ng/mL。它还表现出令人满意的选择性、重现性和稳定性，这为拓展临床FR检测的新途径提供了可能性。