To understand environmental effects affecting paralytic shellfish toxin production of Centrodinium punctatum, this study examined the growth responses, and toxin contents and profiles of a C. punctatum culture exposed to drastic changes of temperature (5-30 °C) and salinity (15–40). C. punctatum grew over a temperature range of 15–25 °C, with an optimum of 20 °C., and over a salinity range of 25–40, with optimum salinities of 30–35. This suggests that C. punctatum prefers relatively warm waters and an oceanic habitat for its growth and can adapt to significant changes of salinity levels. When C. punctatum was cultivated at different temperature and salinity levels, the PST profile included four major analogs (STX, neoSTX, GTX1 and GTX4, constituted >80 % of the profile), while low amounts of doSTX and traces of dc-STX and dc-GTX2 were also observed. Interestingly, though overall toxin contents did not change significantly with temperature, increases in the proportion of STX, and decreases in proportions in GTX1 and GTX4 were observed with higher temperatures. Salinity did not affect either toxin contents or profile from 25 to 35. However, the total toxin content dropped to approximately half at salinity 40, suggesting this salinity may induce metabolic changes in C. punctatum.

为了了解影响麻痹性贝类毒素产生的环境影响Centrodinium punctatum本研究检测了麻痹性贝类的生长反应以及毒素含量和概况。 C. punctatum 培养物暴露于温度 (5-30 °C) 和盐度 (15–40) 的剧烈变化。 C. punctatum的生长温度范围为15-25°C，最适温度为20°C，盐度范围为25-40，最适盐度为30-35。这表明C. punctatum喜欢相对温暖的水域和海洋栖息地生长，能够适应盐度水平的显着变化。当C. punctatum 在不同温度和盐度水平下培养，PST 谱包括四种主要类似物（STX、neoSTX、GTX1 和 GTX4，占谱的 80% 以上），而 doSTX 含量较低还观察到了 dc-STX 和 dc-GTX2 的痕迹。有趣的是，虽然总体毒素含量没有随温度发生显着变化，但随着温度升高，STX 的比例增加，GTX1 和 GTX4 的比例减少。从 25 到 35，盐度不会影响毒素含量或分布。然而，在盐度 40 时，总毒素含量下降到大约一半，表明这种盐度可能会引起 C 的代谢变化。点状体。