5-Aminovaleric acid (5-AVA), 5-hydroxyvalerate (5HV), copolymer P(3HB-co-5HV) of 3-hydroxybutyrate (3HB) and 5HV were produced from L-lysine as a substrate by recombinant Halomonas bluephagenesis constructed based on codon optimization, deletions of competitive pathway and L-lysine export protein, and three copies of davBA genes encoding L-lysine monooxygenase (DavB) and 5-aminovaleramide amidohydrolase (DavA) inserted into its genome to form H. bluephagenesis YF117ΔgabT₁₊₂, which produced 16.4 g L⁻¹ and 67.4 g L⁻¹ 5-AVA in flask cultures and in 7 L bioreactor, respectively. It was able to de novo synthesize 5-AVA from glucose by L-lysine-overproducing H. bluephagenesis TD226. Corn steep liquor was used instead of yeast extract for cost reduction during the 5-AVA production. Using promoter engineering based on P_porin mutant library for downstream genes, H. bluephagenesis YF117 harboring pSEVA341-P_{porin 42}-yqhD_EC produced 6 g L⁻¹ 5HV in shake flask growth, while H. bluephagenesis YF117 harboring pSEVA341-P_{porin 42}-yqhD_EC-P_{porin 278}-phaC_RE-abfT synthesized 42 wt% P(3HB-co-4.8 mol% 5HV) under the same condition. Thus, H. bluephagenesis was successfully engineered to produce 5-AVA and 5HV in supernatant and intracellular P(3HB-co-5HV) utilizing L-lysine as the substrate.

5-氨基戊酸(5-AVA)、5-羟基戊酸(5HV)、3-羟基丁酸(3HB)和5HV的共聚物P(3HB- co -5HV)以L-赖氨酸为底物，通过重组Halomonas bluephagenesis构建密码子优化、竞争途径和 L-赖氨酸输出蛋白的删除，以及将编码 L-赖氨酸单加氧酶 (DavB) 和 5-氨基戊酰胺酰胺水解酶 (DavA) 的三个拷贝的davBA基因插入其基因组中，形成H. bluephagenesis YF117Δ gabT _{1+如图2所示}，其在烧瓶培养物和7L生物反应器中分别产生16.4g L ^-1和67.4g L ^{-1 5-AVA。}它能够通过过量生产 L-赖氨酸的H. bluephagenesis TD226 从葡萄糖合成5-AVA 。在5-AVA生产过程中，使用玉米浆代替酵母提取物以降低成本。使用基于P_孔蛋白突变体库的下游基因启动子工程，携带pSEVA341-P_孔蛋白42 - yqhD _EC的H.bluephagenesis YF117在摇瓶生长中产生6 g L ^{-1 5HV} ，而携带pSEVA341-P_孔蛋白42 - yqhD EC的H.bluephagenesis YF117 yqhD _EC -P_孔蛋白278 - phaC _RE -abfT在相同条件下合成了 42 wt% P(3HB- co -4.8 mol% 5HV)。因此，蓝噬菌发生被成功地改造为利用L-赖氨酸作为底物在上清液和细胞内P(3HB- co -5HV)中产生5-AVA和5HV 。