Biomimetic mineralization of dentin collagen appears to be a promising strategy to optimize dentin bonding durability. However, traditional postbonding mineralization strategies based on Ca/P ion release still have some drawbacks, such as being time-consuming, having a spatiotemporal mismatch, and having limited intrafibrillar minerals. To tackle these problems, a prebonding rapid intrafibrillar mineralization strategy was developed in the present study. Specifically, polyacrylic acid-stabilized amorphous calcium fluoride (PAA-ACF) was found to induce rapid intrafibrillar mineralization of the single-layer collagen model and dentin collagen at just 1 min and 10 min, as identified by transmission electron microscopy, scanning electron microscopy, and atomic force microscopy. This strategy has also been identified to strengthen the mechanical properties of demineralized dentin within a clinically acceptable timeframe. Significantly, the bonding strength of the PAA-ACF-treated groups outperformed the control group irrespective of aging modes. In addition, the endogenous matrix metalloproteinases as well as exogenous bacterial erosion were inhibited, thus reducing the degradation of dentin collagen. High-quality integration of the hybrid layer and the underlying dentin was also demonstrated. On the basis of the present results, the concept of "prebonding rapid intrafibrillar mineralization" was proposed. This user-friendly scheme introduced PAA-ACF-based intrafibrillar mineralization into dentin bonding for the first time. As multifunctional primers, PAA-ACF precursors have the potential to shed new light on prolonging the service life of adhesive restorations, with promising significance.

中文翻译：

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快速纤维内矿化策略增强粘合剂-牙本质界面。

牙本质胶原蛋白的仿生矿化似乎是优化牙本质粘合耐久性的一种有前途的策略。然而，基于Ca/P离子释放的传统键合后矿化策略仍然存在一些缺点，例如耗时、时空不匹配以及原纤维内矿物质有限。为了解决这些问题，本研究开发了一种预粘合快速原纤维内矿化策略。具体而言，通过透射电子显微镜、扫描电子显微镜、和原子力显微镜。该策略还被确定可以在临床可接受的时间内增强脱矿牙本质的机械性能。值得注意的是，无论老化模式如何，PAA-ACF 处理组的粘合强度均优于对照组。此外，内源性基质金属蛋白酶以及外源性细菌侵蚀均受到抑制，从而减少牙本质胶原蛋白的降解。混合层和下面的牙本质的高质量整合也得到了证明。在此基础上，提出了“预结合快速原纤维内矿化”的概念。这种用户友好的方案首次将基于 PAA-ACF 的纤维内矿化引入牙本质粘合中。作为多功能底漆，PAA-ACF前体有可能为延长粘接修复体的使用寿命提供新的思路，具有广阔的前景。