Dental caries is a dynamic disease induced by the unbalance between demineralization of dental hard tissues caused by biofilm and remineralization of them; however, although various effective remineralization methods have been well documented, it is a challenge to reestablish the balance by enhancing remineralization alone while ignoring the antibacterial therapy. Therefore, the integration of remineralizing and antibacterial technologies offers a promising strategy to halt natural caries progression in clinical practice. Here, the conception of interrupting dental caries (IDC) was proposed based on the development of dual-functional coating with remineralizing and antibacterial properties. In this study, bovine serum albumin (BSA) loaded octenidine (OCT) successfully to form a BSA-OCT composite. Subsequently, through fast amyloid-like aggregation, the phase-transited BSA-OCT (PTB-OCT) coating can be covered on teeth, resin composite, or sealant surfaces in 30 min by a simple smearing process. The PTB-OCT coating showed satisfactory effects in promoting the remineralization of demineralized enamel and dentin in vitro. Moreover, this coating also exerted significant acid-resistance stability and anti-biofilm properties. Equally importantly, this coating exhibited promising abilities in reducing the microleakage between the tooth and resin composite in vitro and preventing primary and secondary caries in vivo. In conclusion, this novel dual-functional PTB-OCT coating could reestablish the balance between demineralization and remineralization in the process of caries, thereby potentially preventing or arresting caries.

中文翻译：

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用于阻止龋齿的再矿化和抗菌涂层。

龋齿是由于生物膜引起的牙齿硬组织脱矿与再矿化不平衡而诱发的动态疾病；然而，尽管各种有效的再矿化方法已被充分证明，但仅通过增强再矿化而忽略抗菌治疗来重建平衡是一个挑战。因此，再矿化和抗菌技术的结合为临床实践中阻止自然龋齿的进展提供了一种有前景的策略。在此，基于具有再矿化和抗菌性能的双功能涂层的开发，提出了阻断龋齿（IDC）的概念。在本研究中，牛血清白蛋白（BSA）负载奥替尼啶（OCT）成功形成BSA-OCT复合材料。随后，通过快速的淀粉样蛋白聚集，相变 BSA-OCT (PTB-OCT) 涂层可以通过简单的涂抹过程在 30 分钟内覆盖在牙齿、树脂复合材料或密封剂表面上。PTB-OCT涂层在体外促进脱矿牙釉质和牙本质的再矿化方面显示出令人满意的效果。此外，该涂层还具有显着的耐酸稳定性和抗生物膜性能。同样重要的是，这种涂层在体外减少牙齿和树脂复合材料之间的微渗漏以及在体内预防原发性和继发性龋齿方面表现出良好的能力。总之，这种新型双功能PTB-OCT涂层可以重建龋齿过程中脱矿和再矿化之间的平衡，从而潜在地预防或阻止龋齿。