Fluorescent indicators can provide quantitative insights into the spatiotemporal dynamics of signalling molecules released by brain circuits. However, a mismatch between the experimental context and the experimental imaging settings often introduces unexpected errors and biases in such measurements. Appreciating this mismatch should help researchers to arrive at unbiased estimates. Fluorescent indicators can provide quantitative insights into the spatiotemporal dynamics of signalling molecules released by brain circuits. However, a mismatch between the experimental context and the experimental imaging settings often introduces unexpected errors and biases in such measurements. Appreciating this mismatch should help to arrive at unbiased estimates.

中文翻译：

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避免荧光传感器读数偏差

荧光指示器可以定量了解大脑回路释放的信号分子的时空动态。然而，实验背景和实验成像设置之间的不匹配通常会在此类测量中引入意外的错误和偏差。认识到这种不匹配应该有助于研究人员得出公正的估计。荧光指示器可以定量了解大脑回路释放的信号分子的时空动态。然而，实验背景和实验成像设置之间的不匹配通常会在此类测量中引入意外的错误和偏差。认识到这种不匹配应该有助于得出公正的估计。