Fertilization in mammals is accompanied by an intense period of chromatin remodeling and major changes in nuclear organization. How the earliest events in embryogenesis, including zygotic genome activation (ZGA) during maternal-to-zygotic transition, influence such remodeling remains unknown. Here, we have investigated the establishment of nuclear architecture, focusing on the remodeling of lamina-associated domains (LADs) during this transition. We report that LADs reorganize gradually in two-cell embryos and that blocking ZGA leads to major changes in nuclear organization, including altered chromatin and genomic features of LADs and redistribution of H3K4me3 toward the nuclear lamina. Our data indicate that the rearrangement of LADs is an integral component of the maternal-to-zygotic transition and that transcription contributes to shaping nuclear organization at the beginning of mammalian development.

中文翻译：

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早期小鼠胚胎中核纤层相关结构域的重组受 RNA 聚合酶 II 活性的调节

哺乳动物的受精过程伴随着染色质重塑的强烈时期和核组织的重大变化。胚胎发生中的最早事件，包括母体向合子转变期间的合子基因组激活（ZGA），如何影响这种重塑仍然未知。在这里，我们研究了核结构的建立，重点关注在此转变期间核层相关域（LAD）的重塑。我们报告，LAD 在双细胞胚胎中逐渐重组，并且阻断 ZGA 会导致核组织发生重大变化，包括 LAD 染色质和基因组特征的改变以及 H3K4me3 向核纤层的重新分配。我们的数据表明，LAD 的重排是母体向合子转变的一个组成部分，并且转录有助于在哺乳动物发育初期塑造核组织。