Bacillus sp. is one of the most distinctive gram-positive bacteria, able to grow efficiently using cheap carbon sources and secrete a variety of useful substances, which are widely used in food, pharmaceutical, agricultural and environmental industries. At the same time, Bacillus sp. is also recognized as a safe genus with a relatively clear genetic background, which is conducive to the industrial production of target metabolites. In this review, we discuss the reasons why Bacillus sp. has been so extensively studied and summarize its advances in systems and synthetic biology, engineering strategies to improve microbial cell properties, and industrial applications in several metabolic engineering applications. Finally, we present the current challenges and possible solutions to provide a reliable basis for Bacillus sp. as a microbial cell factory.

芽孢杆菌属。是最具特色的革兰氏阳性菌之一，能够利用廉价的碳源高效生长，并分泌多种有用物质，广泛应用于食品、制药、农业和环境行业。与此同时，芽孢杆菌。也被公认为安全属，具有较为清晰的遗传背景，有利于目标代谢产物的工业化生产。在这篇综述中，我们讨论了芽孢杆菌的原因。已被如此广泛地研究和总结其在系统和合成生物学方面的进展、改善微生物细胞特性的工程策略以及在几种代谢工程应用中的工业应用。最后，我们提出了当前的挑战和可能的解决方案，为芽孢杆菌属提供可靠的基础。作为微生物细胞工厂。