Postnatally, severe vitamin D deficiency commonly results in rickets as well as potential defects in tooth mineralization. The effects of milder deficiency on oral health outcomes later in life are still unclear. This study used micro–computed tomography (μCT), energy dispersive X-ray analysis (EDX), and Raman spectroscopy to investigate mineral density, total density, and elemental composition of enamel and dentine in 63 exfoliated primary incisors from participants with known 25-hydroxyvitamin D levels (25-OHD) at birth. No differences in mineralization and chemical composition using μCT and EDX analysis were observed irrespective of 25-OHD status. Subtle structural differences were observed via Raman spectroscopy, with more crystalline enamel observed in those with sufficient 25-OHD at birth. Although subtle, the differences seen suggest further attention should be given to children with known milder levels of vitamin D deficiency in early life. © 2023 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR).

中文翻译：

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利用实验室分析技术探索乳牙的矿物质密度、元素和化学成分与脐带血维生素 D 的关系

出生后，严重的维生素 D 缺乏通常会导致佝偻病以及牙齿矿化的潜在缺陷。轻度缺乏症对以后生活中口腔健康结果的影响仍不清楚。本研究使用显微计算机断层扫描 (μCT)、能量色散 X 射线分析 (EDX) 和拉曼光谱来研究 63 颗脱落的乳切牙的矿物质密度、总密度以及牙釉质和牙本质的元素组成，这些乳切牙来自已知 25-出生时羟基维生素 D 水平 (25-OHD)。无论 25-OHD 状态如何，使用 μCT 和 EDX 分析均未观察到矿化和化学成分存在差异。通过拉曼光谱观察到细微的结构差异，在出生时具有足够 25-OHD 的人中观察到更多的结晶牙釉质。尽管细微差别，但所观察到的差异表明，应进一步关注早年已知轻度维生素 D 缺乏症的儿童。© 2023 作者。《Journal of Bone and Mineral Research》由 Wiley periodicals LLC 代表美国骨与矿物研究学会 (ASBMR) 出版。