The basidiomycete Serpula lacrymans is responsible for major timber devastation in houses. Basidiomycetes are known to harbour a diverse but poorly understood microbial community of bacteria, archaea, yeasts and filamentous fungi. In this study, we used amplicon-sequencing to analyse the abundance and composition of prokaryotic communities associated with fruiting bodies of S. lacrymans and compared them to communities of surrounding material to access the ‘background’ community structure. Our findings indicate that bacterial genera cluster depended on sample type and that the main driver for microbial diversity is specimen, followed by sample origin. The most abundant bacterial phylum identified in the fruiting bodies was Pseudomonadota, followed by Actinomycetota and Bacteroidota. The prokaryote community of the mycelium was dominated by Actinomycetota, Halobacterota and Pseudomonadota. Actinomycetota was the most abundant phylum in both environment samples (infested timber and underground scree), followed by Bacillota in wood and Pseudomonadota in underground samples. Nocardioides, Pseudomonas, Pseudonochardia, Streptomyces and Rubrobacter spp. were among others found to comprise the core microbiome of S. lacrymans basidiocarps. This research contributes to the understanding of the holobiont S. lacrymans and gives hints to potential bacterial phyla important for its development and lifestyle.

中文翻译：

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与不同类型的组织形成相关的原核生物群落和 Serpula lacrymans 栖息的基质

担子菌Serpula lacrymans是造成房屋木材严重破坏的罪魁祸首。众所周知，担子菌含有多种但人们知之甚少的微生物群落，包括细菌、古细菌、酵母和丝状真菌。在这项研究中，我们使用扩增子测序来分析与S. lacrymans子实体相关的原核生物群落的丰度和组成，并将它们与周围材料的群落进行比较，以了解“背景”群落结构。我们的研究结果表明，细菌属簇取决于样本类型，微生物多样性的主要驱动因素是样本，其次是样本来源。子实体中最丰富的细菌门是假单胞菌门，其次是放线菌门和拟杆菌门。菌丝体的原核生物群落以放线菌门、盐杆菌门和假单胞菌门为主。放线菌门是两种环境样本（受感染的木材和地下碎石）中最丰富的门，其次是木材中的杆菌门和地下样本中的假单胞菌门。诺卡氏菌属、假单胞菌属、假诺卡氏菌属、链霉菌属和红杆菌属。等被发现构成了S. lacrymans basidiocarps 的核心微生物组。这项研究有助于了解全生物S. lacrymans，并为对其发育和生活方式重要的潜在细菌门提供线索。