Microeukaryotes are key for predicting the change of ecosystem processes in the face of a disturbance. However, their vertical responses to multiple interconnected factors caused by water mixing remain unknown. Here, we conducted a 12-month high-frequency study to compare the impacts of mixing disturbances on microeukaryotic community structure and stability over different depths in a stratified reservoir. We demonstrate that core and satellite microeukaryotic compositions and interactions in surface waters were not resistant to water mixing, but significantly recovered. This was because the water temperature rebounded to the pre-mixing level. Core microeukaryotes maintained community stability in surface waters with high recovery capacity after water mixing. In contrast, the changes in water temperature, chlorophyll-a, and nutrients resulted in steep and prolonged variations in the bottom core and satellite microeukaryotic compositions and interactions. Under low environmental fluctuation, the recovery of microbial communities did not affect nutrient cycling in surface waters. Under high environmental fluctuation, core and satellite microeukaryotic compositions in bottom waters were significantly correlated with the multi-nutrient cycling index. Our findings shed light on different mechanisms of plankton community resilience in reservoir ecosystems to a major disturbance over depths, highlighting the role of bottom microeukaryotes in nutrient cycling.

中文翻译：

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完全混合后核心类群驱动深亚热带水库微真核生物群落稳定性

微真核生物是预测生态系统过程在受到干扰时发生变化的关键。然而，它们对水混合引起的多个相互关联因素的垂直响应仍然未知。在这里，我们进行了一项为期 12 个月的高频研究，比较混合干扰对分层水库不同深度的微真核生物群落结构和稳定性的影响。我们证明，地表水中的核心和卫星微真核生物组成和相互作用不抵抗水混合，但显着恢复。这是因为水温回升至混合前的水平。核心微真核生物在地表水中保持群落稳定性，在水混合后具有较高的恢复能力。相比之下，水温、叶绿素a和营养物质的变化导致底部核心和卫星微真核生物的组成和相互作用发生急剧且长期的变化。在环境波动较小的情况下，微生物群落的恢复不影响地表水的养分循环。在高环境波动下，底水核心和卫星微真核生物组成与多养分循环指数显着相关。我们的研究结果揭示了水库生态系统中浮游生物群落对深度重大扰动的恢复力的不同机制，强调了底部微真核生物在营养循环中的作用。