The present study evaluated the therapeutic potential of the medicinal plant Lysimachia candida Lindl. against metabolic syndrome in male SD rats fed with a high-fat high-fructose (HFHF) diet. Methanolic extract of Lysimachia candida Lindl. (250 mg kg⁻¹ body weight p.o.) was administrated to the HFHF-fed rats daily for 20 weeks. Blood samples were collected, and blood glucose levels and relevant biochemical parameters were analysed and used for the assessment of metabolic disease phenotypes. In this study, Lysimachia candida decreased HFHF diet-induced phenotypes of metabolic syndrome, i.e., obesity, blood glucose level, hepatic triglycerides, free fatty acids, and insulin resistance. Liquid chromatography-mass spectrometry-based metabolomics was done to study the dynamics of metabolic changes in the serum during disease progression in the presence and absence of the treatment. Furthermore, multivariate data analysis approaches have been employed to identify metabolites responsible for disease progression. Lysimachia candida Lindl. plant extract restored the metabolites that are involved in the biosynthesis and degradation of amino acids, fatty acid metabolism and vitamin metabolism. Interestingly, the results depicted that the treatment with the plant extract restored the levels of acetylated amino acids and their derivatives, which are involved in the regulation of beta cell function, glucose homeostasis, insulin secretion, and metabolic syndrome phenotypes. Furthermore, we observed restoration in the levels of indole derivatives and N-acetylgalactosamine with the treatment, which indicates a cross-talk between the gut microbiome and the metabolic syndrome. Therefore, the present study revealed the potential mechanism of Lysimachia candida Lindl. extract to prevent metabolic syndrome in rats.

中文翻译：

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非靶向代谢组学和表型数据表明了念珠菌的治疗和预防潜力。针对高脂肪高果糖诱导的大鼠代谢综合征

本研究评估了药用植物珍珠菜的治疗潜力。对抗高脂高果糖（HFHF）饮食的雄性 SD 大鼠的代谢综合征。珍珠菜的甲醇提取物。(250 mg kg ^-1体重 po) 每天给予 HFHF 喂养的大鼠，持续 20 周。采集血液样本，分析血糖水平和相关生化参数，用于代谢疾病表型的评估。在这项研究中，念珠菌降低了 HFHF 饮食诱导的代谢综合征表型，即肥胖、血糖水平、肝甘油三酯、游离脂肪酸和胰岛素抵抗。采用基于液相色谱-质谱法的代谢组学来研究在存在或不存在治疗的情况下疾病进展期间血清代谢变化的动态。此外，多变量数据分析方法已被用来识别导致疾病进展的代谢物。念珠菌Lindl。植物提取物恢复了参与氨基酸生物合成和降解、脂肪酸代谢和维生素代谢的代谢物。有趣的是，结果表明，用植物提取物治疗可以恢复乙酰化氨基酸及其衍生物的水平，这些氨基酸参与调节β细胞功能、葡萄糖稳态、胰岛素分泌和代谢综合征表型。此外，我们观察到治疗后吲哚衍生物和N-乙酰半乳糖胺的水平恢复，这表明肠道微生物组和代谢综合征之间存在相互作用。因此，本研究揭示了珍珠菜的潜在机制。提取物可预防大鼠代谢综合征。