Abstract

Conventional treatments for tumors were frequently accompanied by drawbacks and side effects. It might be useful to use the revolutionary microneedle technology which combines photothermal therapy with tumor immunotherapy. In this study, we created a microneedle drug delivery system with mercapto-modified gold nanorods and immune checkpoint blocker anti-PD-1 polypeptide. With good mechanical strength, the microneedle system can efficiently penetrate the skin and deliver drugs. When inserted into human skin, anti-PD-1 peptides and gold nanorods can be released, boosting the capacity of cytotoxic T lymphocytes to destroy tumor cells. Additionally, the elimination of the tumor is aided by the production of heat while being exposed to near-infrared light. This microneedle drug delivery system can enhance the immunological reaction and prolong the survival time of mice. Moreover, it has been demonstrated that the system has mild toxic and side effects on normal tissues and can effectively inhibit the growth of tumors, indicating a bright prospect for the treatment of cancers.

摘要

肿瘤的常规治疗经常伴随着缺点和副作用。使用将光热疗法与肿瘤免疫疗法相结合的革命性微针技术可能会有用。在这项研究中，我们创建了一种含有巯基修饰的金纳米棒和免疫检查点阻断剂抗PD-1多肽的微针药物递送系统。微针系统具有良好的机械强度，可以有效地穿透皮肤并输送药物。当插入人体皮肤时，可以释放抗PD-1肽和金纳米棒，增强细胞毒性T淋巴细胞破坏肿瘤细胞的能力。此外，暴露在近红外光下时产生的热量有助于消除肿瘤。这种微针给药系统可以增强免疫反应，延长小鼠的生存时间。而且已证明该系统对正常组织毒副作用轻微，能有效抑制肿瘤的生长，在癌症治疗方面具有广阔的前景。