Electroconductive biomaterials are gaining significant consideration for regeneration in tissues where electrical functionality is of crucial importance, such as myocardium, neural, musculoskeletal, and bone tissue. In this work, conductive biohybrid platforms were engineered by blending collagen type I and 2D MXene (Ti₃C₂T_x) and afterwards covalently crosslinking; to harness the biofunctionality of the protein component and the increased stiffness and enhanced electrical conductivity (matching and even surpassing native tissues) that two-dimensional titanium carbide provides. These MXene platforms were highly biocompatible and resulted in increased proliferation and cell spreading when seeded with fibroblasts. Conversely, they limited bacterial attachment (Staphylococcus aureus) and proliferation. When neonatal rat cardiomyocytes (nrCMs) were cultured on the substrates increased spreading and viability up to day 7 were studied when compared to control collagen substrates. Human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) were seeded and stimulated using electric-field generation in a custom-made bioreactor. The combination of an electroconductive substrate with an external electrical field enhanced cell growth, and significantly increased cx43 expression. This in vitro study convincingly demonstrates the potential of this engineered conductive biohybrid platform for cardiac tissue regeneration.

导电生物材料在电功能至关重要的组织（例如心肌、神经、肌肉骨骼和骨组织）的再生中得到了广泛的关注。在这项工作中，导电生物混合平台是通过混合 I 型胶原蛋白和 2D MXene (Ti ₃ C ₂ T _x)，然后共价交联；利用二维碳化钛提供的蛋白质成分的生物功能以及增加的刚度和增强的导电性（匹配甚至超越天然组织）。这些 MXene 平台具有高度生物相容性，当接种成纤维细胞时，会导致增殖和细胞扩散增加。相反，它们限制细菌附着（金黄色葡萄球菌）和增殖。当新生大鼠心肌细胞 (nrCM) 在基质上培养时，与对照胶原蛋白基质相比，研究发现直至第 7 天的扩散和活力均有所增加。在定制的生物反应器中使用电场生成来接种和刺激人类诱导多能干细胞衍生的心肌细胞 (iPSC-CM)。导电基质与外部电场的结合增强了细胞生长，并显着增加了 cx43 表达。这项体外研究令人信服地证明了这种工程传导性生物混合平台在心脏组织再生方面的潜力。