Spectral microscopy provides information about the spatial distribution and physiological functional states of pigment-protein complexes in photosynthetic organisms. This can be used to complement the newly developed techniques, such as cryogenic electron microscopy and atomic force microscopy, which are less effective in functional analysis of photosynthesis, despite having an excellent spatial resolution. The combination of optical microscopies with various spectroscopic techniques has extended the possibility of a multi-perspective investigation in photosynthesis research. Some of these spectroscopic techniques include fluorescence and absorption spectra, excitation spectra, time-resolved fluorescence measurement, Raman scattering spectroscopy, etc. These techniques can be applied to in vivo investigations of photosynthetic activity without introducing any artificial fluorophore since the photosynthetic pigments are informative probes. In particular, the technique has been effective in clarifying the dynamic physiological responses of photosynthetic organisms to variable environments. In this paper, we review the recent progress in spectral microscopy in the field of in vivo photosynthesis research. We have also introduced and discussed some distinctive spectral microscopies such as anti-Stokes fluorescence spectral microscopy, excitation spectral microscopy, cryo-microscopy, and Raman spectral microscopy.

光谱显微镜提供有关光合生物中色素-蛋白质复合物的空间分布和生理功能状态的信息。这可以用来补充新开发的技术，例如低温电子显微镜和原子力显微镜，尽管它们具有出色的空间分辨率，但在光合作用的功能分析中效果较差。光学显微镜与各种光谱技术的结合扩展了光合作用研究多视角研究的可能性。其中一些光谱技术包括荧光和吸收光谱、激发光谱、时间分辨荧光测量、拉曼散射光谱等。这些技术可以应用于光合活性的体内研究，而无需引入任何人工荧光团，因为光合色素是信息探针。特别是，该技术可以有效地阐明光合生物对可变环境的动态生理反应。在本文中，我们回顾了光谱显微镜在体内光合作用研究领域的最新进展。我们还介绍并讨论了一些独特的光谱显微镜，例如反斯托克斯荧光光谱显微镜、激发光谱显微镜、冷冻显微镜和拉曼光谱显微镜。该技术有效地阐明了光合生物对可变环境的动态生理反应。在本文中，我们回顾了光谱显微镜在体内光合作用研究领域的最新进展。我们还介绍并讨论了一些独特的光谱显微镜，例如反斯托克斯荧光光谱显微镜、激发光谱显微镜、冷冻显微镜和拉曼光谱显微镜。该技术有效地阐明了光合生物对可变环境的动态生理反应。在本文中，我们回顾了光谱显微镜在体内光合作用研究领域的最新进展。我们还介绍并讨论了一些独特的光谱显微镜，例如反斯托克斯荧光光谱显微镜、激发光谱显微镜、冷冻显微镜和拉曼光谱显微镜。