Comparative Characterization of the Ginsenosides from Six Panax Herbal Extracts and Their In Vitro Rat Gut Microbial Metabolites by Advanced Liquid Chromatography–Mass Spectrometry Approaches,Journal of Agricultural and Food Chemistry

当前位置： X-MOL 学术 › J. Agric. Food Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Comparative Characterization of the Ginsenosides from Six Panax Herbal Extracts and Their In Vitro Rat Gut Microbial Metabolites by Advanced Liquid Chromatography–Mass Spectrometry Approaches
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-06-09 , DOI: 10.1021/acs.jafc.3c01093
Yue-Guang Mi _{1,

2} , Xiao-Yan Xu _{1,

2} , Li-Li Hong _{1,

2} , Mei-Ting Jiang _{1,

2} , Bo-Xue Chen _{1,

2} , Xiao-Hang Li _{1,

2} , Hong-da Wang _{1,

2} , Ya-Dan Zou _{1,

2} , Xin Zhao _{1,

2} , Zheng Li _{1,

2,

3} , De-An Guo _{1,

2,

4} , Wen-Zhi Yang _{1,

2}

Affiliation

Ginseng extracts are extensively used as raw materials for food supplements and herbal medicines. This study aimed to characterize ginsenosides obtained from six Panax plant extracts (Panax ginseng, red ginseng, Panax quinquefolius, Panax notoginseng, Panax japonicus, and Panax japonicus var. major) and compared them with their in vitro metabolic profiles mediated by rat intestinal microbiota. Ultrahigh-performance liquid chromatography/ion mobility–quadrupole time-of-flight mass spectrometry (UHPLC/IM-QTOF-MS) with scheduled multiple reaction monitoring (sMRM) quantitation methods were developed to characterize and compare the ginsenoside composition of the different extracts. After in vitro incubation, 248 ginsenosides/metabolites were identified by UHPLC/IM-QTOF-MS in six biotransformed samples. Deglycosylation was determined to be the main metabolic pathway of ginsenosides, and protopanaxadiol-type and oleanolic acid-type saponins were easier to be easily metabolized. Compared with the ginsenosides in plant extracts, those remaining in six biotransformed samples were considerably fewer after biotransformation for 8 h. However, the compositional differences in four subtypes of the ginsenosides among the six Panax plants became more distinct.

中文翻译：

通过高级液相色谱-质谱法对六种人参草药提取物中的人参皂苷及其体外大鼠肠道微生物代谢物进行比较表征

人参提取物广泛用作食品补充剂和草药的原料。本研究旨在表征从六种人参植物提取物（人参、红参、西洋参、三七、日本人参和大参）中提取的人参皂苷，并与体外试验的人参皂苷进行比较。由大鼠肠道微生物群介导的代谢特征。开发了超高效液相色谱/离子淌度-四极杆飞行时间质谱 (UHPLC/IM-QTOF-MS) 和预定多反应监测 (sMRM) 定量方法来表征和比较不同提取物的人参皂苷成分。体外后孵育后，通过 UHPLC/IM-QTOF-MS 在 6 个生物转化样品中鉴定出 248 种人参皂苷/代谢物。确定去糖基化是人参皂苷的主要代谢途径，原人参二醇型和齐墩果酸型皂苷更容易被代谢。与植物提取物中的人参皂苷相比，生物转化8小时后，6个生物转化样品中残留的人参皂苷明显减少。然而，六种人参植物中人参皂苷的四种亚型的组成差异变得更加明显。

更新日期：2023-06-09

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11