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Correction to “Novel Peptides Derived from Sea Cucumber Intestine Promotes Osteogenesis by Upregulating Integrin-Mediated Transdifferentiation of Growth Plate Chondrocytes to Osteoblasts”
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-06-08 , DOI: 10.1021/acs.jafc.3c02613
Hao Yue , Yingying Tian , Xiaoxuan Feng , Yuying Bo , Changhu Xue , Ping Dong , Jingfeng Wang

Figure 4 and Figure 6 as originally published were incorrect. The correct figures are given below. Page 13213, second paragraph, left column: delete “osteoporotic” in second to last sentence. Page 13214, second paragraph, right column: change third and fourth sentences to “Among the five fractions, SCIP-P3 possessed the highest osteogenic activity (29.21% increased in 50 μg/mL, p < 0.01, Figure 1E). In particular, Figure 1F,G also demonstrated that SCIP-P3 significantly increased the mineralized nodules of MC3T3-E1 cells by 2.11 fold (p < 0.01).” Page 13217, second paragraph, right column: change “Igtb1” in the last sentence to “Itgb1”. Page 13218, first paragraph, left column: change second sentence to “In addition, the SCIP supplement downregulated the expression of histone methyltransferases Smyd1 and upregulated the expression of histone methyltransferases Kmt5c and Kmt2d, and histone demethylases Kdm6a and Kdm1a.” Page 13218, second paragraph, left column: change “As shown in Figure 5C,D” in second sentence to “As shown in Figure 6D,E.” Page 13218, second paragraph, left column: change third and fourth sentences to “In addition, the gene expressions Prkcg, Ccnd1, Prkaca, Sfrp2, Porcn, Dvl3, Fzd7, Sfrp4, Gsk3β, Ror2, LRP5, and Wnt10b in Wnt signaling were significantly upregulated by the SCIP supplement, whereas the expression of Wnt4, Fosl1 and Dkk1 were downregulated (Figure 6F). Western blot results further showed that the SCIP supplement significantly upregulated the expression of p-GSK3β, and enhanced the accumulation of β-catenin in the nucleus (Figure 6G).” Figure 2. Caption, (F) and (G) interchange. The authors sincerely apologize for the inconvenience that this causes to the journal and the stakeholders. Figure 4. Effect of SCIP on the key gene expression in the adolescent mice growth plate. (A) PCA analysis of samples from different experimental groups; (B) volcano plot of gene expression difference between SCIP group and normal group; (C) gene expression of the marker during the chondrocyte to osteoblast transdifferentiation; (D) western bolt analysis for osteoprogenitor cells and bone mesenchymal stem cell markers. Data are expressed as mean ± SD (n = 6). *p < 0.05, **p < 0.01 (vs the normal group). Figure 6. Effect of SCIP on integrin-mediated histone modifications and Wnt signaling in the adolescent mice growth plate. (A) Gene expression of histone modification-related enzymes; (B) gene expression of pluripotent transcription factors; (C) western bolt analysis for histone acetylation and pluripotent transcription factors; (D) western bolt analysis for integrins; (E) growth plate Wnt10b level; (F) gene expression of the Wnt/β-catenin signaling pathway; (G) western bolt analysis for the Wnt/β-catenin signaling pathway. Data are expressed as mean ± SD (n = 6). *p < 0.05, **p < 0.01 (vs the normal group). This article has not yet been cited by other publications. Figure 4. Effect of SCIP on the key gene expression in the adolescent mice growth plate. (A) PCA analysis of samples from different experimental groups; (B) volcano plot of gene expression difference between SCIP group and normal group; (C) gene expression of the marker during the chondrocyte to osteoblast transdifferentiation; (D) western bolt analysis for osteoprogenitor cells and bone mesenchymal stem cell markers. Data are expressed as mean ± SD (n = 6). *p < 0.05, **p < 0.01 (vs the normal group). Figure 6. Effect of SCIP on integrin-mediated histone modifications and Wnt signaling in the adolescent mice growth plate. (A) Gene expression of histone modification-related enzymes; (B) gene expression of pluripotent transcription factors; (C) western bolt analysis for histone acetylation and pluripotent transcription factors; (D) western bolt analysis for integrins; (E) growth plate Wnt10b level; (F) gene expression of the Wnt/β-catenin signaling pathway; (G) western bolt analysis for the Wnt/β-catenin signaling pathway. Data are expressed as mean ± SD (n = 6). *p < 0.05, **p < 0.01 (vs the normal group).

中文翻译:

更正“海参肠衍生的新肽通过上调整合素介导的生长板软骨细胞向成骨细胞的转分化来促进成骨”

最初发布的图 4 和图 6 不正确。下面给出了正确的数字。第13213页,第二段,左栏:删除倒数第二句中的“骨质疏松症”。第 13214 页,第二段,右栏:将第三句和第四句更改为“在五个组分中,SCIP-P3 具有最高的成骨活性(50 μg/mL 时增加 29.21%,p < 0.01,图 1E 。特别是,图1F,G还表明SCIP-P3显着增加了MC3T3-E1细胞的矿化结节2.11倍(p< 0.01)。” 第13217页,第二段,右栏:将最后一句中的“Igtb1”更改为“Itgb1”。第 13218 页,第一段,左栏:将第二句更改为“此外,SCIP 补充剂下调组蛋白甲基转移酶 Smyd1 的表达,上调组蛋白甲基转移酶 Kmt5c 和 Kmt2d 以及组蛋白去甲基酶 Kdm6a 和 Kdm1a 的表达。” 第13218页,第二段,左栏:将第二句中的“如图5C,D所示”更改为“如图6D,E所示”。第13218页,第二段,左栏:将第三句和第四句改为“此外,Wnt信号传导中的基因表达Prkcg、Ccnd1、Prkaca、Sfrp2、Porcn、Dvl3、Fzd7、Sfrp4、Gsk3β、Ror2、LRP5和Wnt10b为SCIP 补充剂显着上调,而 Wnt4 的表达,Fosl1 和 Dkk1 下调(图 6F)。蛋白质印迹结果进一步表明,SCIP 补充剂显着上调 p-GSK3β 的表达,并增强 β-catenin 在细胞核中的积累(图 6G)。” 图 2. 标题、(F) 和 (G) 互换。对于由此给期刊和利益相关者带来的不便,作者深表歉意。图 4. SCIP 对青春期小鼠生长板关键基因表达的影响。(A) 不同实验组样本的PCA分析;(B) SCIP组与正常组基因表达差异火山图;(C)软骨细胞向成骨细胞转分化过程中标记物的基因表达;(D) 骨祖细胞和骨间充质干细胞标记物的蛋白质螺栓分析。数据表示为平均值±SD ( n = 6)。* p < 0.05,** p< 0.01(与正常组相比)。图 6. SCIP 对青春期小鼠生长板中整合素介导的组蛋白修饰和 Wnt 信号传导的影响。(A) 组蛋白修饰相关酶的基因表达;(B)多能转录因子的基因表达;(C) 组蛋白乙酰化和多能转录因子的蛋白质印迹分析;(D) 整合素的 Western Bolt 分析;(E)生长板Wnt10b水平;(F) Wnt/β-连环蛋白信号通路的基因表达;(G) Wnt/β-连环蛋白信号通路的蛋白质螺栓分析。数据表示为平均值±SD ( n = 6)。* p < 0.05,** p< 0.01(与正常组相比)。这篇文章尚未被其他出版物引用。图 4. SCIP 对青春期小鼠生长板关键基因表达的影响。(A) 不同实验组样本的PCA分析;(B) SCIP组与正常组基因表达差异火山图;(C)软骨细胞向成骨细胞转分化过程中标记物的基因表达;(D) 骨祖细胞和骨间充质干细胞标记物的蛋白质螺栓分析。数据表示为平均值±SD ( n = 6)。* p < 0.05,** p< 0.01(与正常组相比)。图 6. SCIP 对青春期小鼠生长板中整合素介导的组蛋白修饰和 Wnt 信号传导的影响。(A) 组蛋白修饰相关酶的基因表达;(B)多能转录因子的基因表达;(C) 组蛋白乙酰化和多能转录因子的蛋白质印迹分析;(D) 整合素的 Western Bolt 分析;(E)生长板Wnt10b水平;(F) Wnt/β-连环蛋白信号通路的基因表达;(G) Wnt/β-连环蛋白信号通路的蛋白质螺栓分析。数据表示为平均值±SD ( n = 6)。* p < 0.05,** p < 0.01(与正常组相比)。
更新日期:2023-06-08
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