Pasteurization is an economical and convenient method to eliminate harmful microorganisms in raw materials for fermentation. However, the surviving species in pasteurized wheat straw and their potential effects on white rot fungi fermentation remain unclear. In this study, wheat straw was pasteurized at different temperatures (60–85 ℃) and fermented with Irpex lacteus for 28 days. At the end of fermentation, the acid-insoluble lignin (ADL) content decreased significantly in all pasteurized samples (P < 0.05). We found a negative correlation between ADL content and pasteurization temperature (R =0.992, P < 0.001). In enzymatic hydrolysis, the sugar yield of samples pasteurized at 80 ℃ (T80) was higher 39.9% than that at 60 ℃. Regarding microbial community, I. lacteus was the dominant fungus in T80 at an abundance of 93.4%, whereas the abundance of other fungi, such as Chaetomium and Aspergillus, was below 1.60%. Three bacteria, including Bacillus, Lysinibacillus, and Paenibacillus, had a combined abundance of 80.5% in T80, which could be attributed to their high-temperature tolerance and symbiosis with I. lacteus. Bacterial functions, such as nitrogen cycling, cellulolysis, chitinolysis, and methylotrophy, were enriched in T60 samples, but the functions and those related to human and animal pathogen reduced in T80 samples, which promoted the growth and degradation of I. lacteus. Overall, thermotolerant fungi and spore-forming bacterial species are the dominant microorganisms in wheat straw pasteurized above 80 ℃, which promotes the colonization and degradation of I. lacteus and improves the biosafety of treated wheat straw.

巴氏杀菌法是一种经济、简便的消除发酵原料中有害微生物的方法。然而，巴氏杀菌小麦秸秆中的存活物种及其对白腐真菌发酵的潜在影响仍不清楚。在这项研究中，小麦秸秆在不同温度（60-85℃）下进行巴氏杀菌，并用Irpex lacteus发酵28 天。在发酵结束时，所有巴氏杀菌样品的酸不溶性木质素（ADL）含量均显着降低（P  < 0.05）。我们发现 ADL 含量与巴氏杀菌温度呈负相关（R = 0.992，P  < 0.001）。在酶水解，80℃（T80）巴氏杀菌样品的糖得率比60℃高39.9％。关于微生物群落， I. lacteus是 T80 中的优势真菌，丰度为 93.4%，而其他真菌（如毛壳菌和曲霉菌）的丰度低于 1.60%。Bacillus、 Lysinibacillus和Paenibacillus三种细菌在 T80 中的总丰度为 80.5% ，这可能归因于它们的高温耐受性和与I. lacteus的共生. 氮循环、纤维素分解、几丁质分解和甲基营养等细菌功能在T60样品中富集，但在T80样品中功能和与人和动物病原体相关的功能减少，促进了I. lacteus的生长和降解。总体而言，耐热真菌和产芽孢菌种是80℃以上巴氏杀菌小麦秸秆中的优势微生物，促进了I. lacteus的定植和降解，提高了经处理小麦秸秆的生物安全性。