Constructing an Intensified UDP Recycling System for the Glycosylation of Natural Products by Phosphorylation of Byproduct Fructose,Journal of Agricultural and Food Chemistry

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Constructing an Intensified UDP Recycling System for the Glycosylation of Natural Products by Phosphorylation of Byproduct Fructose
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-06-08 , DOI: 10.1021/acs.jafc.3c01077
Yingying Wang ₁ , Qiuyan Sun ₁ , Yanfei Chi ₁ , Zhiqiang Liu ₁ , Hu Liu ₁ , Chun Li _{1,

2} , Xudong Feng ₁

Affiliation

UDP-glucosyltransferase can be coupled with sucrose synthase to construct a two-enzyme UDP (UDP-2E) recycling system for glucosylation of natural products with inexpensive sucrose as the consumed substrate. However, sucrose hydrolysis leads to the accumulation of fructose as a byproduct, which decreases the atom economy of sucrose and suppresses in situ UDP recycling. In this study, a polyphosphate-dependent glucokinase was demonstrated to convert fructose to fructose-6-phosphate independent of expensive ATP for the first time. Then the glucokinase was introduced into the UDP-2E recycling system to construct a modified three-enzyme UDP (UDP-3E) recycling system, which showed enhanced glucosylation efficiency of triterpenoids by fructose phosphorylation to accelerate sucrose hydrolysis and UDP recycling. Finally, by further introducing a phosphofructokinase into the UDP-3E recycling system, we transformed fructose-6-phosphate into fructose-1,6-diphosphate, demonstrating that the UDP-3E recycling system can be coupled with extra enzymes to obtain final products with high added-value without compromising the glycosylation efficiency.

中文翻译：

副产物果糖磷酸化天然产物糖基化强化UDP回收体系

UDP-葡萄糖基转移酶可以与蔗糖合酶偶联，构建双酶UDP（UDP-2E）回收系统，以廉价的蔗糖作为消耗底物对天然产物进行糖基化。然而，蔗糖水解导致副产物果糖的积累，这降低了蔗糖的原子经济性并抑制了原位UDP回收。在这项研究中，首次证明多磷酸盐依赖性葡萄糖激酶可以将果糖转化为果糖-6-磷酸，而不依赖于昂贵的 ATP。然后将葡萄糖激酶引入UDP-2E回收系统，构建改良的三酶UDP（UDP-3E）回收系统，该系统通过果糖磷酸化提高三萜类化合物的糖基化效率，加速蔗糖水解和UDP回收。最后，

更新日期：2023-06-08

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