Enzyme, produced and worked in all living things, could work as macromolecular biological catalysts in diverse biochemical processes with particular specificity, like glucose oxidase (GO_X). The efficient use of enzyme properties has great importance in pharmaceutics and therapeutics. In this work, we could fabricate naive and effective electrochemical biosensors in the determination of glucose levels via utilizing GOx. Graphene oxide, as a water-soluble derivative of graphene, has shown great promise in a variety of biomedical applications including biosensors. Thus, we established a new-type special platform for GOx immobilization to perform its prosperities, in which nanographene oxide (nGO) was employed as an ideal base and poly(ethylene glycol) (PEG) was conjugated on the edge of nGO sheets to enhance its biocompatibility. Additionally, preferable functional dyes (Rhodamine B/fluorescein isothiocyanate) were also introduced to the platform. Enzyme-nanocomposites were then provided by locating GO_X on the platform, i.e., GO_X@nGO-PEG-RhB and GO_X@nGO-PEG-FITC. The microstructure and composite of platforms and enzyme-nanocomposites were confirmed by diverse characterizations. Finally, on account of corresponding cyclic voltammetric and typical ready-state amperometric curves, it was informed that GO_X@nGO-PEG-RhB and GO_X@nGO-PEG-FITC could effectively respond to the fluctuation of glucose level as electrochemical biosensor. The present work presents special platforms for the immobilization of enzymes like GO_X and provides new-type biosensors in the detection of glucose levels.

在所有生物体中产生和发挥作用的酶可以在多种生化过程中作为大分子生物催化剂发挥作用，具有特殊的特异性，例如葡萄糖氧化酶 (GO _X )。酶特性的有效利用在药物学和治疗学中具有重要意义。在这项工作中，我们可以通过利用 GO x来制造简单且有效的电化学生物传感器来测定葡萄糖水平。氧化石墨烯作为石墨烯的水溶性衍生物，在包括生物传感器在内的各种生物医学应用中显示出巨大的潜力。因此，我们为GO x建立了一个新型的专用平台固定化以发挥其优势，其中纳米氧化石墨烯（nGO）被用作理想的基础，聚（乙二醇）（PEG）被缀合在nGO片材的边缘以增强其生物相容性。此外，还向平台引入了优选的功能性染料（罗丹明 B/异硫氰酸荧光素）。然后通过在平台上定位 GO _X提供酶纳米复合材料，即 GO _X @nGO-PEG-RhB 和 GO _X @nGO-PEG-FITC。平台和酶-纳米复合材料的微观结构和复合材料通过多种表征得到证实。最后，根据相应的循环伏安曲线和典型的准备状态电流曲线，得知 GO _X @nGO-PEG-RhB 和 GO _X@nGO-PEG-FITC 作为电化学生物传感器可以有效响应葡萄糖水平的波动。目前的工作为固定酶（如 GO _X）提供了特殊平台，并提供了检测葡萄糖水平的新型生物传感器。