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Cloning and functional characterization of a tau class glutathione transferase associated with haloxyfop-P-methyl resistance in Digitaria sanguinalis
Pest Management Science ( IF 4.1 ) Pub Date : 2023-05-29 , DOI: 10.1002/ps.7588
Xiangying Liu 1, 2 , Zhenlin Hou 1 , Yuying Zhang 1 , Austin Merchant 3 , Mei-E Zhong 4 , Guolan Ma 2 , Qing Zeng 2 , Lamei Wu 2 , Xuguo Zhou 3 , Kun Luo 1 , Chunxia Ding 1, 4
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Haloxyfop-P-methyl, an acetyl-CoA carboxylase (ACCase)-inhibiting herbicide, has been extensively used to control grass weeds. Widespread use of haloxyfop-P-methyl in cotton fields in China has led to the development of glutathione transferase (GST)-mediated resistance in Digitaria sanguinalis. An RNA-seq analysis identified DsGSTU1, a tau class glutathione transferase from the D. sanguinalis transcriptome as a potential candidate. Here, we cloned DsGSTU1 from D. sanguinalis young leaf tissues and subsequently characterized DsGSTU1 by a combination of sequence analysis, as well as functional heterologous expression in rice.
更新日期:2023-05-29
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