As reservoirs of pathogens, antimicrobial resistant microorganisms and a wide variety of pollutants, hospital wastewaters (HWWs) need to be effectively treated before discharge. This study employed the functionalized colloidal microbubble technology as one-step fast HWW treatment. Inorganic coagulant (monomeric Fe(III)-coagulant or polymeric Al(III)-coagulant) and ozone were used as surface-decorator and gaseous core modifier, respectively. The Fe(III)- or Al(III)-modified colloidal gas (or, ozone) microbubbles (Fe(III)-CCGMBs, Fe(III)-CCOMBs, Al(III)-CCGMBs and Al(III)-CCOMBs) were constructed. Within 3 min, CCOMBs decreased COD_Cr and fecal coliform concentration to the levels meeting the national discharge standard for medical organization. Regrowth of bacteria was inhibited and biodegradability of organics was increased after the simultaneous oxidation and cell-inactivation process. The metagenomics analysis further reveals that Al(III)-CCOMBs performed best in capturing the virulence genes, antibiotic resistance genes and their potential hosts. The horizontal transfer of those harmful genes could be effectively hampered thanks to the removal of mobile genetic elements. Interestingly, the virulence factors of adherence, micronutrient uptake/acquisition and phase invasion could facilitate the interface-dominated capture. Featured as cascade processes of capture, oxidation and inactivation in the one-step operation, the robust Al(III)-CCOMB treatment is recommended for the HWW treatment and the protection of downstream aquatic environment.

医院废水 (HWW) 是病原体、抗菌药物耐药微生物和多种污染物的储存库，在排放前需要进行有效处理。本研究采用功能化胶体微泡技术作为一步式快速 HWW 处理。无机混凝剂（单体Fe(III)-混凝剂或聚合Al(III)-混凝剂）和臭氧分别用作表面装饰剂和气态核心改性剂。Fe(III)-或Al(III)-改性胶体气体（或臭氧）微泡（Fe(III)-CCGMB、Fe(III)-CCOMB、Al(III)-CCGMB 和 Al(III)-CCOMB)被建造。3 分钟内，CCOMB 降低了 COD _Cr粪便大肠菌群浓度达到国家医疗机构排放标准。同时氧化和细胞灭活过程后，细菌的再生受到抑制，有机物的生物降解性增加。宏基因组学分析进一步表明，Al(III)-CCOMBs 在捕获毒力基因、抗生素抗性基因及其潜在宿主方面表现最佳。由于移动遗传元件的去除，这些有害基因的水平转移可以得到有效阻碍。有趣的是，粘附、微量营养素摄取/获取和相侵入的毒力因子可以促进界面主导的捕获。其特点是一步操作中捕获、氧化和失活的级联过程，