Amniotic membrane (AM) has great potential as a scaffold for tissue regeneration in reconstructive surgery. To date, no systematic review of the literature has been performed for the applications of AM in wound closure of internal organs. Therefore, in this systematic review and meta-analysis, we summarize the literature on the safety and efficacy of AM for the closure of internal organs. A systematic search was performed in MEDLINE-PubMed database and OVID Embase to retrieve human and controlled animal studies on wound closure of internal organs. The Cochrane Risk of Bias tool for randomized clinical trials and the SYRCLE risk of bias tool for animal studies were used. Meta-analyses (MAs) were conducted for controlled animal studies to assess efficacy of closure, mortality and complications in subjects who underwent surgical wound closure in internal organs with the application of AM. Sixty references containing 26 human experiments and 36 animal experiments were included. The MAs of the controlled animal studies showed comparable results with regard to closure, mortality and complications, and suggested improved mechanical strength and lower inflammation scores after AM application when compared to standard surgical closure techniques. This systematic review and MAs demonstrate that the application of AM to promote wound healing of internal organs appears to be safe, efficacious, and feasible.

中文翻译：

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羊膜在内脏器官重建手术中的应用——系统评价和Meta分析

羊膜 (AM) 作为重建手术中组织再生的支架具有巨大潜力。迄今为止，尚未对 AM 在内部器官伤口闭合中的应用进行系统的文献回顾。因此，在本系统评价和荟萃分析中，我们总结了有关 AM 用于关闭内脏器官的安全性和有效性的文献。在 MEDLINE-PubMed 数据库和 OVID Embase 中进行了系统搜索，以检索关于内脏伤口闭合的人类和对照动物研究。使用了用于随机临床试验的 Cochrane 偏倚风险工具和用于动物研究的 SYRCLE 偏倚风险工具。对受控动物研究进行荟萃分析 (MAs) 以评估闭合效果，通过应用 AM 在内脏器官进行手术伤口闭合的受试者的死亡率和并发症。包括 60 个参考文献，其中包含 26 个人体实验和 36 个动物实验。受控动物研究的 MAs 在闭合、死亡率和并发症方面显示了可比较的结果，并表明与标准手术闭合技术相比，应用 AM 后机械强度提高，炎症评分降低。该系统评价和 MA 表明应用 AM 促进内脏器官伤口愈合似乎是安全、有效和可行的。受控动物研究的 MAs 在闭合、死亡率和并发症方面显示了可比较的结果，并表明与标准手术闭合技术相比，应用 AM 后机械强度提高，炎症评分降低。该系统评价和 MA 表明应用 AM 促进内脏器官伤口愈合似乎是安全、有效和可行的。受控动物研究的 MAs 在闭合、死亡率和并发症方面显示了可比较的结果，并表明与标准手术闭合技术相比，应用 AM 后机械强度提高，炎症评分降低。该系统评价和 MA 表明应用 AM 促进内脏器官伤口愈合似乎是安全、有效和可行的。