Biofilm formation on an implant surface is most commonly caused by the human pathogenic bacteria Staphylococcus aureus, which can lead to implant related infections and failure. It is a major problem for both implantable orthopedic and maxillofacial devices. The current antibiotic treatments are typically delivered orally or in an injectable form. They are not highly effective in preventing or removing biofilms, and they increase the risk of antibiotic resistance of bacteria and have a dose-dependent negative biological effect on human cells. Our aim was to improve current treatments via a localized and controlled antibiotic delivery-based implant coating system to deliver the antibiotic, gentamicin (Gm). The coating contains coral skeleton derived hydroxyapatite powders (HAp) that act as antibiotic carrier particles and have a biodegradable poly-lactic acid (PLA) thin film matrix. The system is designed to prevent implant related infections while avoiding the deleterious effects of high concentration antibiotics in implants on local cells including primary human adipose derived stem cells (ADSCs). Testing undertaken in this study measured the rate of S. aureus biofilm formation and determined the growth rate and proliferation of ADSCs. After 24 h, S. aureus biofilm formation and the percentage of live cells found on the surfaces of all 5%–30% (w/w) PLA-Gm-(HAp-Gm) coated Ti6Al4V implants was lower than the control samples. Furthermore, Ti6Al4V implants coated with up to 10% (w/w) PLA-Gm-(HAp-Gm) did not have noticeable Gm related adverse effect on ADSCs, as assessed by morphological and surface attachment analyses. These results support the use and application of the antibacterial PLA-Gm-(HAp-Gm) thin film coating design for implants, as an antibiotic release control mechanism to prevent implant-related infections.

中文翻译：

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在 Ti6Al4V 植入物上结合抗生素负载珊瑚生物陶瓷的聚乳酸涂层对金黄色葡萄球菌的体外测试和功效

植入物表面的生物膜形成最常见的原因是人类致病菌金黄色葡萄球菌，这可能导致植入物相关感染和失败。对于植入式骨科和颌面设备来说，这是一个主要问题。目前的抗生素治疗通常是口服或注射形式。它们在预防或去除生物膜方面不是很有效，而且会增加细菌产生抗生素耐药性的风险，并对人体细胞产生剂量依赖性的负面生物学效应。我们的目标是通过局部和受控的基于抗生素输送的种植体涂层系统来输送抗生素庆大霉素 (Gm)，从而改进当前的治疗方法。该涂层包含珊瑚骨架衍生的羟基磷灰石粉末 (HAp)，作为抗生素载体颗粒，并具有可生物降解的聚乳酸 (PLA) 薄膜基质。该系统旨在防止植入物相关感染，同时避免植入物中高浓度抗生素对局部细胞（包括原代人脂肪干细胞 (ADSC)）的有害影响。本研究中进行的测试测量了年代。金黄色葡萄球菌生物膜形成并确定 ADSCs 的生长速率和增殖。24 小时后，S。在所有 5%–30% (w/w) PLA-Gm-(HAp-Gm) 涂层 Ti6Al4V 植入物的表面上发现的金黄色葡萄球菌生物膜形成和活细胞百分比低于对照样品。此外，通过形态学和表面附着分析评估，涂有高达 10% (w/w) PLA-Gm-(HAp-Gm) 的 Ti6Al4V 植入物对 ADSC 没有明显的 Gm 相关不利影响。这些结果支持植入物的抗菌 PLA-Gm-(HAp-Gm) 薄膜涂层设计的使用和应用，作为预防植入物相关感染的抗生素释放控制机制。