The Gram-positive bacterium Bacillus subtilis produces a diverse range of secondary metabolites with different structures and activities. Among them, bacilysin is an enzymatically synthesized dipeptide that consists of L-alanine and L-anticapsin. Previous research by our group has suggested bacilysin's role as a pleiotropic molecule in its producer, B. subtilis PY79. However, the nature of protein interactions in the absence of bacilysin has not been defined. In the present work, we constructed a protein-protein interaction subnetwork by using Omics Integrator based on our recent comparative proteomics data obtained from a bacilysin-silenced strain, OGU1. Functional enrichment analyses on the resulting networks pointed to certain putatively perturbed pathways such as citrate cycle, quorum sensing and secondary metabolite biosynthesis. Various molecules, which were absent from the experimental data, were included in the final network. We believe that this study can guide further experiments in the identification and confirmation of protein-protein interactions in B. subtilis.

中文翻译：

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作为枯草芽孢杆菌中杆菌溶素生物合成功能的差异表达蛋白质的比较生物网络分析。

革兰氏阳性细菌枯草芽孢杆菌产生多种具有不同结构和活性的次级代谢物。其中，杆菌肽是一种酶促合成的二肽，由L-丙氨酸和L-抗辣椒素组成。我们小组先前的研究表明，杆菌溶素在其生产者枯草芽孢杆菌 PY79 中作为多效分子的作用。然而，在没有杆菌溶素的情况下蛋白质相互作用的性质尚未确定。在目前的工作中，我们基于我们最近从杆菌溶素沉默菌株 OGU1 获得的比较蛋白质组学数据，使用 Omics Integrator 构建了蛋白质-蛋白质相互作用子网络。对所得网络的功能富集分析指出了某些假定的扰动途径，例如柠檬酸循环、群体感应和次生代谢物生物合成。实验数据中不存在的各种分子被包含在最终网络中。我们相信这项研究可以指导进一步的实验，以识别和确认枯草芽孢杆菌中的蛋白质-蛋白质相互作用。