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Enzymatic hydrolysis at high dry matter content: The influence of the substrates’ physical properties and of loading strategies on mixing and energetic consumption
Bioresource Technology ( IF 11.4 ) Pub Date : 2017-11-20 , DOI: 10.1016/j.biortech.2017.11.049
Federico Battista , Mélanie Gomez Almendros , Romain Rousset , Serge Boivineau , Pierre-Antoine Bouillon

The present work investigates the impact of the physical properties and loading strategies of wheat straw and miscanthus on enzymatic hydrolysis at high DM concentration. Three parameters have been chosen to evaluate the enzymatic hydrolysis performance: (i) the mixing time, (ii) the energetic mixing consumption and (iii) the glucose concentration. It was demonstrated that the hydrolysis of miscanthus is easy to perform and has low viscosity. On the contrary, the higher porosity grade of wheat straw than miscanthus (73% against 52%) contributed to have a very high viscosity at 20% w/w DM. The development of a fed-batch strategy allowed the reduction of viscosity inducing the energetic consumption lowering from 30 kJ to 10 kJ. It has been also proven that the miscanthus addition in wheat straw achieved to decrease mixing energy consumption at 5–8 kJ, when it represented more than 30% of the total mass of the reaction medium.



中文翻译:

高干物质含量下的酶水解:底物的物理性质和加载策略对混合和能量消耗的影响

本工作研究了高DM浓度下小麦秸秆和桔属植物的物理性质和负载策略对酶促水解的影响。选择了三个参数来评估酶促水解性能:(i)混合时间,(ii)能量混合消耗和(iii)葡萄糖浓度。结果表明,梅花的水解容易进行且粘度低。相反,小麦秸秆的孔隙度等级高于桔梗(73%对52%),从而在20%w / w时具有非常高的粘度DM。分批补料策略的发展允许降低粘度,从而将能量消耗从30 kJ降低到10 kJ。还已经证明,当麦草中的黄花菜占反应介质总质量的30%以上时,在5–8 kJ时降低了混合能量的消耗就可以了。

更新日期:2017-11-20
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