Herein, we present an overview of recent research progress in the development of micro/nanoelectrochemical probe and chip devices for the evaluation of three-dimensional (3D) cultured cells. First, we discuss probe devices: a general outline, evaluation of O₂ consumption, enzyme-modified electrodes, evaluation of endogenous enzyme activity, and the collection of cell components from cell aggregates are discussed. The next section is focused on integrated chip devices: a general outline, electrode array devices, smart electrode array devices, droplet detection of 3D cultured cells, cell manipulation using dielectrophoresis (DEP), and electrodeposited hydrogels used for fabrication of 3D cultured cells on chip devices are discussed. Finally, we provide a summary and discussion of future directions of research in this field.

中文翻译：

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用于评估三维培养细胞的微/纳米电化学探针和芯片装置

在此，我们概述了用于评估三维（3D）培养细胞的微/纳米电化学探针和芯片设备的最新研究进展。首先，我们讨论探针设备：概述，O ₂评估消耗，酶修饰的电极，内源性酶活性的评估，以及从细胞聚集体中收集细胞成分进行了讨论。下一部分的重点是集成芯片设备：概述，电极阵列设备，智能电极阵列设备，3D培养细胞的液滴检测，使用介电电泳（DEP）的细胞操作以及用于在芯片上制造3D培养细胞的电沉积水凝胶讨论了设备。最后，我们对该领域的未来研究方向进行了总结和讨论。