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Tip-enhanced Raman spectroscopy of amyloid β at neuronal spines
Analyst ( IF 3.6 ) Pub Date : 2017-10-20 00:00:00 , DOI: 10.1039/c7an00744b Mohammadali Tabatabaei 1, 2, 3, 4 , Fabiana A. Caetano 2, 3, 5, 6 , Farshid Pashee 1, 2, 3, 4 , Stephen S. G. Ferguson 7, 8, 9, 10 , François Lagugné-Labarthet 1, 2, 3, 4
Analyst ( IF 3.6 ) Pub Date : 2017-10-20 00:00:00 , DOI: 10.1039/c7an00744b Mohammadali Tabatabaei 1, 2, 3, 4 , Fabiana A. Caetano 2, 3, 5, 6 , Farshid Pashee 1, 2, 3, 4 , Stephen S. G. Ferguson 7, 8, 9, 10 , François Lagugné-Labarthet 1, 2, 3, 4
Affiliation
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The early stages of Alzheimer's disease pathogenesis are thought to occur at the synapse level, since synapse loss can be directly correlated with memory dysfunction. Considerable evidence has suggested that amyloid beta (Aβ), a secreted proteolytic derivative of amyloid precursor protein, appears to be a critical factor in the early ‘synaptic failure’ that is observed in Alzheimer's disease pathogenesis. The identification of Aβ at neuronal spines with high spatial resolution and high surface specificity would facilitate unraveling the intricate effect of Aβ on synapse loss and its effect on neighboring neuronal connections. Here, tip-enhanced Raman spectroscopy was used to map the presence of Aβ aggregations in the vicinity of the spines exposed to Aβ preformed in vitro. Exposure to Aβ was of 1 and 6 hours. The intensity variation of selected vibrational modes of Aβ was mapped by TERS for different exposure times to Aβ. Of interest, we discuss the distinct contributions of the amide modes from Aβ that are enhanced by the TERS process and in particular the suppression of the amide I mode in the context of recently reported observations in the literature.
中文翻译：
神经元棘的淀粉样β的尖端增强拉曼光谱
人们认为，阿尔茨海默氏病发病机理的早期阶段发生在突触水平，因为突触丢失可能与记忆功能障碍直接相关。大量证据表明，淀粉样蛋白前体蛋白的一种分泌蛋白水解衍生物淀粉样蛋白β（Aβ）似乎是阿尔茨海默氏病发病机理中早期“突触衰竭”的关键因素。在具有高空间分辨率和高表面特异性的神经元棘突中鉴定Aβ将有助于阐明Aβ对突触损失的复杂作用及其对邻近神经元连接的作用。在这里，尖端增强拉曼光谱用于绘制暴露于体外预先形成的Aβ的棘突附近Aβ聚集的存在情况。暴露于Aβ分别为1小时和6小时。对于不同的暴露时间，通过TERS绘制了选定的Aβ振动模式的强度变化图。有趣的是，我们讨论了通过TERS过程增强的Aβ酰胺模式的独特贡献，特别是在最近报道的文献报道的背景下，酰胺I模式的抑制。
更新日期：2017-11-20
中文翻译：
神经元棘的淀粉样β的尖端增强拉曼光谱
人们认为，阿尔茨海默氏病发病机理的早期阶段发生在突触水平，因为突触丢失可能与记忆功能障碍直接相关。大量证据表明，淀粉样蛋白前体蛋白的一种分泌蛋白水解衍生物淀粉样蛋白β（Aβ）似乎是阿尔茨海默氏病发病机理中早期“突触衰竭”的关键因素。在具有高空间分辨率和高表面特异性的神经元棘突中鉴定Aβ将有助于阐明Aβ对突触损失的复杂作用及其对邻近神经元连接的作用。在这里，尖端增强拉曼光谱用于绘制暴露于体外预先形成的Aβ的棘突附近Aβ聚集的存在情况。暴露于Aβ分别为1小时和6小时。对于不同的暴露时间，通过TERS绘制了选定的Aβ振动模式的强度变化图。有趣的是，我们讨论了通过TERS过程增强的Aβ酰胺模式的独特贡献，特别是在最近报道的文献报道的背景下，酰胺I模式的抑制。
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