A new lateral-flow immunochromatographic strip combined with quantum dot nanobeads and gold nanoflowers for rapid detection of tetrodotoxin,Analyst

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A new lateral-flow immunochromatographic strip combined with quantum dot nanobeads and gold nanoflowers for rapid detection of tetrodotoxin
Analyst ( IF 3.6 ) Pub Date : 2017-10-11 00:00:00 , DOI: 10.1039/c7an01227f
Haicong Shen _{1,

2,

3,

4,

5} , Fei Xu _{1,

2,

3,

4,

5} , Meng Xiao _{1,

2,

3,

4,

5} , Qiangqiang Fu _{1,

2,

3,

4,

5} , Zhenzhu Cheng _{5,

6,

7,

8} , Shiwei Zhang _{9,

10,

11,

12} , Caihong Huang _{1,

2,

3,

4,

5} , Yong Tang _{1,

2,

3,

4,

5}

Affiliation

Tetrodotoxin (TTX) is a potent, low molecular weight analyte that can lead to fatal poisoning and requires a sensitive, rapid detection method. Here, we have developed a competitive, lateral-flow immunochromatographic strip combined with quantum dot nanobeads (QDNBs) and gold nanoflowers (AuNFs). This approach is called turn-on C-LFICS and it meets all testing requirements. Subsequent analysis revealed that this turn-on C-LFICS was rapid (8 min), sensitive (LOD = 0.2 ng mL⁻¹), and quantitative (DLR = 1.56–100 ng mL⁻¹), and had a positive signal readout (based on fluorescence quenching effects) for TTX detection. Moreover, it had superior signal brightness and a low background interference signal when compared with previous methods. Finally, it can function free of interference from the sample matrix and has a demonstrated recovery range of 85.5% to 119.7% in spiked samples. Taken together, these results show that our turn-on C-LFICS is an effective detection tool for TTX or other small molecules.

中文翻译：

结合量子点纳米珠和金纳米花的新型侧流免疫层析带可快速检测河豚毒素

河豚毒素（TTX）是一种有效的低分子量分析物，可导致致命的中毒，需要一种灵敏，快速的检测方法。在这里，我们开发了一种竞争性的侧流免疫色谱带，结合了量子点纳米珠（QDNB）和金纳米花（AuNFs）。这种方法称为开启式C-LFICS，它可以满足所有测试要求。随后的分析表明，这种开启的C-LFICS快速（8分钟），灵敏（LOD = 0.2 ng mL ^-1）和定量（DLR = 1.56–100 ng mL ^-1）），并且具有用于TTX检测的正信号读数（基于荧光猝灭效应）。此外，与以前的方法相比，它具有出色的信号亮度和较低的背景干扰信号。最后，它的功能不受样品基质的干扰，加标样品的回收率范围为85.5％至119.7％。综上所述，这些结果表明，我们的C-LFICS接通电源是检测TTX或其他小分子的有效工具。

更新日期：2017-11-20

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