A porous boron-doped diamond (PBDD)/Ta sensing electrode was prepared for the fast, sensitive, stable, and discriminative detection of dopamine (DA) and pyridoxine (vitamin B6) in human serum. All the exposed surfaces of the diamond grains of the BDD layer were etched into a porous form, with pore sizes of less than 500 nm and an average depth of about 200 nm. The electrochemical performance characteristics of the PBDD layer and the reaction mechanisms enabling the detection of DA and vitamin B6 were studied. Large numbers of oxygen-containing groups on the PBDD surface, as well as the activity difference of the different planes, enabled us to successfully distinguish between DA and B6 by using the PBDD/Ta electrode. The low background current of PBDD, the large active area of the porous surface, and the high electron transfer properties led to the PBDD electrode having a high sensitivity. Therefore, this sensor can be used to stably detect DA and B6 in serum. Aberrant levels of DA and vitamin B6 in body fluids are key risk indicators for some diseases; thus, monitoring the levels of both and other species in serum is of great significance to clinical diagnoses.

制备了多孔硼掺杂金刚石（PBDD）/ Ta感应电极，用于快速，灵敏，稳定和区分性检测人血清中的多巴胺（DA）和吡ido醇（维生素B6）。将BDD层的金刚石晶粒的所有暴露表面蚀刻成多孔形式，其孔径小于500nm，平均深度为约200nm。研究了PBDD层的电化学性能特征以及能够检测DA和维生素B6的反应机理。PBDD表面上的大量含氧基团以及不同平面的活性差异使我们能够通过使用PBDD / Ta电极成功区分DA和B6。PBDD的背景电流低，多孔表面的有效面积大，高的电子传递性能导致PBDD电极具有高灵敏度。因此，该传感器可用于稳定地检测血清中的DA和B6。体液中DA和维生素B6的异常水平是某些疾病的关键风险指标；因此，监测血清中两种及其他物种的水平对临床诊断具有重要意义。