A novel electrochemical sensing platform based on combination of multi-walled carbon nanotubes and copper-nickel hybrid nanoparticles ([email protected]/MWCNTs) was developed for simultaneous detection of guanine (G) and adenine (A). The Ni/MWCNTs and [email protected]/MWCNTs nanocomposites were characterized by transmission electron microscopy (TEM) and energy-dispersive X-ray spectroscopy (EDS). The electrochemical behaviors of G and A on the modified electrode were explored by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) in phosphate buffer with pH 3.0. Under the optimal conditions, electrical signals were linear over the concentration ranges from 5.0 to 180 μM and 8.0 to 150 μM for simultaneous determination G and A with the detection limit as low as 0.35 μM and 0.56 μM (S/N = 3), respectively. Furthermore, linear concentration ranges in individual determination are 1.0–180 μM and 2.0–150 μM with detection limits of 0.17 μM and 0.33 μM (S/N = 3) for G and A, respectively. The sensor was successfully used to quantify G and A in real samples. The [email protected]/MWCNTs composite presented here can serve as a promising candidate for developing electrochemical sensor devices and plays an important role in widespread fields.

开发了一种基于多壁碳纳米管和铜-镍杂化纳米粒子（[电子邮件保护] / MWCNTs）结合的新型电化学传感平台，用于同时检测鸟嘌呤（G）和腺嘌呤（A）。Ni / MWCNTs和[受电子邮件保护] / MWCNTs纳米复合材料的特征在于透射电子显微镜（TEM）和能量色散X射线光谱（EDS）。通过循环伏安法（CV）和微分脉冲伏安法（DPV）在pH 3.0的磷酸盐缓冲液中研究了修饰电极上G和A的电化学行为。在最佳条件下，电信号在5.0至180μM和8.0至150μM的浓度范围内呈线性，用于同时测定G和A，检测限分别低至0.35μM和0.56μM（S / N = 3） 。此外，单个测定中的线性浓度范围为1.0–180μM和2.0–150μM，G和A的检出限分别为0.17μM和0.33μM（S / N = 3）。该传感器已成功用于定量真实样品中的G和A。本文介绍的[受电子邮件保护] / MWCNTs复合材料可作为开发电化学传感器设备的有前途的候选者，并在广泛领域中发挥重要作用。