A sensitive and specific method, utilising high performance liquid chromatography tandem mass spectrometry (HPLC–MS/MS) was developed for the quantitative determination of amlodipine in dried blood spot (DBS) samples. Chromatographic separation was achieved using a Waters XBridge C18 column with gradient elution of a mixture of water and acetonitrile containing 0.1% formic acid (v/v). Amlodipine was quantified using a Waters Quattro Premier mass spectrometer coupled with an electro-spray ionization (ESI) source in positive ion mode. The MRM transitions of 408.9 m/z → 238.1 m/z and 408.9 → 294.0 m/z were used to quantify and qualify amlodipine, respectively. The method was validated across the concentration range of 0.5–30 ng/mL by assessing specificity, sensitivity, linearity, precision, accuracy, recovery and matrix effect according to the Food and Drug Administration (FDA) guidelines. This method was also validated clinically within a large pharmacoepidemiological study in which amlodipine blood concentration was determined in patients who had been prescribed this medication.

建立了一种灵敏且特异的方法，利用高效液相色谱串联质谱法（HPLC-MS / MS）定量测定干血斑（DBS）样品中的氨氯地平。使用Waters XBridge C18色谱柱进行色谱分离，梯度洗脱的是水和含0.1％甲酸（v / v）的乙腈的混合物。使用Waters Quattro Premier质谱仪和正离子模式下的电喷雾电离（ESI）源对氨氯地平进行定量。MRM过渡为408.9  m / z  →238.1  m / z和408.9→294.0  m / z分别用于定量和鉴定氨氯地平。根据食品和药物管理局（FDA）指南评估特异性，灵敏度，线性，精密度，准确性，回收率和基质效应，该方法在0.5–30 ng / mL的浓度范围内得到验证。该方法还在大型药物流行病学研究中得到了临床验证，该研究确定了服用该药物的患者的氨氯地平血药浓度。