In this work, a high-sensitive electrochemical sensor of Norfloxacin (NFX) has been developed using a composite electrode of MWCNT-CPE/pRGO-ANSA/Au. MWCNT-CPE is the multi-walled carbon nanotubes (MWCNT) – carbon paste electrode (CPE). pRGO-ANSA means the partially reduced graphene oxide (pRGO) – 6-aminonaphthalene-2-sulphonic acid (ANSA) hybrid nanomaterials. Au represents gold nanoparticles. The electrochemical sensing of NFX was investigated by cyclic voltammetry (CV), linear sweep voltammetry (LSV) and differential pulse voltammetry (DPV). Experimental results show that the combination of MWCNT-CPE/pRGO-ANSA/Au offers high electrical conductivity, large surface area and excellent electrocatalytic activity, being beneficial for electrochemical sensing performance. The calibration curves of NFX exhibit linear response in the NFX concentration range of 0.03–1.0 and 1.0–50.0 μM, with the limit of detection (LOD) of 0.016 μM (S/N = 3). The fabricated sensors were successfully applied for detecting the presence of NFX in pharmaceutical formulations and rat plasma samples. The measured results were comparable with those obtained using ultrahigh performance liquid chromatography (UHPLC), indicating its high potential for applications in pharmaceutical analysis and clinical therapeutic drug monitoring.

在这项工作中，诺氟沙星（NFX）的高灵敏度电化学传感器已经开发出来，使用的是MWCNT-CPE / pRGO-ANSA / Au复合电极。MWCNT-CPE是多壁碳纳米管（MWCNT）–碳糊电极（CPE）。pRGO-ANSA是指部分还原的氧化石墨烯（pRGO）– 6-氨基萘-2-磺酸（ANSA）杂化纳米材料。Au表示金纳米颗粒。通过循环伏安法（CV），线性扫描伏安法（LSV）和差分脉冲伏安法（DPV）研究了NFX的电化学传感。实验结果表明，MWCNT-CPE / pRGO-ANSA / Au的组合具有较高的电导率，较大的表面积和优异的电催化活性，有利于电化学传感性能的提高。NFX的校准曲线在NFX浓度范围为0.03–1.0和1.0–50.0μM时表现出线性响应，检出限（LOD）为0.016μM（S / N = 3）。所制造的传感器已成功应用于检测药物制剂和大鼠血浆样品中NFX的存在。测量结果与使用超高效液相色谱（UHPLC）获得的结果相当，表明其在药物分析和临床治疗药物监测中的应用潜力很大。