The risk of developing hypersensitivity to alternative antibiotics is a concern for penicillin hypersensitive patients and healthcare providers. Herein we use piperacillin hypersensitivity as a model to explore the reactivity of drug-specific IgG against alternative β-lactam protein adducts. Mass spectrometry was used to show the drugs (amoxicillin, flucloxacillin, benzyl penicillin, aztreonam, and piperacillin) bind to similar lysine residues on the protein carrier bovine serum albumin. However, the hapten-specific IgG antibodies found in piperacillin hypersensitive patient plasma did not bind to other β-lactam protein conjugates. These data outline the fine specificity of piperacillin-specific IgG antibodies that circulate in patients with hypersensitivity.

中文翻译：

---

抗哌拉西林 IgG 与结构相关药物蛋白加合物结合的评估

对替代抗生素产生过敏的风险是青霉素过敏患者和医疗保健提供者关心的问题。在这里，我们使用哌拉西林超敏反应作为模型来探索药物特异性 IgG 对替代 β-内酰胺蛋白加合物的反应性。质谱法用于显示药物（阿莫西林、氟氯西林、苄基青霉素、氨曲南和哌拉西林）与蛋白质载体牛血清白蛋白上的相似赖氨酸残基结合。然而，在哌拉西林过敏患者血浆中发现的半抗原特异性 IgG 抗体不与其他 β-内酰胺蛋白偶联物结合。这些数据概述了在过敏患者中循环的哌拉西林特异性 IgG 抗体的良好特异性。