Genome editing using engineered nucleases (meganucleases, zinc finger nucleases, transcription activator-like effector nucleases) has created many recent breakthroughs. Prescreening for efficiency and specificity is a critical step prior to using any newly designed genome editing tool for experimental purposes. The current standard screening methods of evaluation are based on DNA sequencing or use mismatch-sensitive endonucleases. They can be time-consuming and costly or lack reproducibility. Here, we review and critically compare standard techniques with those more recently developed in terms of reliability, time, cost, and ease of use.

中文翻译：

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评估基因组编辑有效性的技术比较

使用工程化核酸酶（大范围核酸酶、锌指核酸酶、转录激活子样效应核酸酶）进行基因组编辑最近取得了许多突破。在使用任何新设计的基因组编辑工具进行实验之前，预筛选效率和特异性是关键步骤。目前的标准筛选评估方法基于 DNA 测序或使用错配敏感的核酸内切酶。它们可能既耗时又昂贵，或者缺乏可重复性。在这里，我们从可靠性、时间、成本和易用性方面回顾并严格比较标准技术与最近开发的技术。