A previous report demonstrated that EuDO3A could be used as an NMR shift reagent for imaging extracellular lactate produced by cancer cells using CEST imaging. In this work, a series of heptadentate macrocyclic YbDO3A-trisamide complexes with δ-chiral carbons in the three pendant side-arms were examined as shift reagents for lactate detection. High resolution ¹H NMR spectra and DFT calculations provided evidence for the formation of stereoselective lactate·YbDO3A-trisamide complexes each with a different CEST signature. This stereoselectivity allowed discrimination of d- versus l-lactate by both high-resolution NMR and CEST. This work demonstrates that lanthanide-based paramagnetic shift reagents can be designed to detect important metabolites by CEST MRI selectively.

中文翻译：

---

CEST在顺磁性位移试剂的帮助下对d和l乳酸酯的对映体识别

先前的报告表明EuDO3A可用作NMR移位试剂，用于使用CEST成像对癌细胞产生的细胞外乳酸进行成像。在这项工作中，研究了三个悬垂侧臂中带有δ手性碳的一系列七齿大环YbDO3A-三酰胺配合物作为乳酸检测的转移试剂。高分辨率的¹ H NMR光谱和DFT计算为形成立体选择性的乳酸·YbDO3A-三酰胺复合物提供了证据，每种复合物具有不同的CEST标记。这种立体选择性可以区分d-与l-通过高分辨率NMR和CEST进行乳酸酯化。这项工作表明，可以设计基于镧系元素的顺磁转移试剂，以通过CEST MRI选择性检测重要的代谢物。