The ecological effects of interacting stressors within lotic ecosystems have been widely acknowledged. In particular, the ecological effects of elevated fine sediment inputs and phosphate have been identified as key factors influencing faunal community structure and composition. However, while knowledge regarding adult and larval life stage responses to environmental stressors has grown, there has been very limited research on their eggs. In this study, the eggs of the mayfly Serratella ignita (Ephemerellidae: Ephemeroptera) were collected and incubated in laboratory aquaria to hatching under differing concentrations of inert suspended sediment (SS) and orthophosphate (OP), individually and in combination. Results indicate that SS and OP have greater effects on egg hatching in combination than when either were considered in isolation. SS displayed a greater effect on egg survival than OP in isolation or when OP was added to elevated SS treatments. Egg mortality in control treatments was around 6% compared to 45% in treatments with 25 mg l⁻¹ SS and 52% in 0.3 mg l⁻¹ OP treatments. Even relatively modest levels of each stressor (10 mg l⁻¹ SS; 0.1 mg l⁻¹ OP), below national legal thresholds, had significant effects on egg survival to hatching. The results support calls for legal levels of SS to be reassessed and suggest that more research is required to assess the impacts of pollution on invertebrate egg development given their different sensitivity and exposure pathways compared to other life stages.

抽水生态系统中相互作用的应激源对生态的影响已得到广泛认可。特别是，高细泥沙输入和磷酸盐的生态效应已被确定为影响动物群落结构和组成的关键因素。然而，尽管关于成年和幼虫生命阶段对环境压力的反应的知识已经增长，但对它们的卵的研究却非常有限。在这项研究中，may蝇卵的卵收集（Ephemerellidae：Ephemeroptera），并在实验室水族箱中分别或组合在不同浓度的惰性悬浮沉淀物（SS）和正磷酸盐（OP）下孵化孵化。结果表明，与单独考虑时相比，SS和OP对蛋孵化的影响更大。与单独使用OP或将SS添加到提高的SS处理中相比，SS对卵存活率显示出更大的影响。对照处理中的鸡蛋死亡率约为6％，而25 mg l ^-1 SS的处理中鸡蛋死亡率为45 ％，0.3 mg l ^-1 OP的处理中鸡蛋率为52％。每个应激源的水平相对较低（10 mg l ^-1 SS; 0.1 mg l ^-1低于国家法律规定的阈值，对卵到孵化的存活率有重大影响。研究结果支持要求重新评估SS的法律水平，并建议需要更多的研究来评估污染对无脊椎动物卵发育的影响，因为它们与其他生命阶段相比具有不同的敏感性和暴露途径。