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Sequencing the Dermatan Sulfate Chain of Decorin
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2017-11-13 00:00:00 , DOI: 10.1021/jacs.7b10164 Yanlei Yu 1 , Jiana Duan 2 , Franklin E. Leach 2 , Toshihiko Toida 3 , Kyohei Higashi 3 , Hong Zhang 1 , Fuming Zhang , I. Jonathan Amster 2 , Robert J. Linhardt
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2017-11-13 00:00:00 , DOI: 10.1021/jacs.7b10164 Yanlei Yu 1 , Jiana Duan 2 , Franklin E. Leach 2 , Toshihiko Toida 3 , Kyohei Higashi 3 , Hong Zhang 1 , Fuming Zhang , I. Jonathan Amster 2 , Robert J. Linhardt
Affiliation
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Glycomics represents one of the last frontiers and most challenging in omic analysis. Glycosylation occurs in the endoplasmic reticulum and the Golgi organelle and its control is neither well-understood nor predictable based on proteomic or genomic analysis. One of the most structurally complex classes of glycoconjugates is the proteoglycans (PGs) and their glycosaminoglycan (GAG) side chains. Previously, our laboratory solved the structure of the chondroitin sulfate chain of the bikunin PG. The current study examines the much more complex structure of the dermatan sulfate GAG chain of decorin PG. By utilizing sophisticated separation methods followed by compositional analysis, domain mapping, and tandem mass spectrometry coupled with analysis by a modified genetic algorithm approach, the structural motif for the decorin dermatan sulfate chain was determined. This represents the second example of a GAG with a prominent structural motif, suggesting that the structural variability of this class of glycoconjugates is somewhat simpler than had been expected.
中文翻译：
测序Decorin硫酸皮肤素链
糖脂学是眼科学分析中最前沿的领域之一，也是最具挑战性的领域。糖基化发生在内质网和高尔基细胞器中，基于蛋白质组学或基因组分析，其控制既不容易理解，也不可预测。糖缀合物中结构最复杂的一类是蛋白聚糖（PGs）及其糖胺聚糖（GAG）侧链。以前，我们的实验室解决了比库宁PG的硫酸软骨素链的结构。当前的研究检查了decorin PG的硫酸皮肤素GAG链的复杂得多的结构。通过利用复杂的分离方法，然后进行成分分析，域映射和串联质谱分析，再结合改进的遗传算法方法进行的分析，确定了玉米芯蛋白硫酸皮肤素链的结构基序。这代表了具有突出的结构基序的GAG的第二个例子，表明这类糖缀合物的结构变异性比预期的要简单一些。
更新日期：2017-11-16
中文翻译：
测序Decorin硫酸皮肤素链
糖脂学是眼科学分析中最前沿的领域之一，也是最具挑战性的领域。糖基化发生在内质网和高尔基细胞器中，基于蛋白质组学或基因组分析，其控制既不容易理解，也不可预测。糖缀合物中结构最复杂的一类是蛋白聚糖（PGs）及其糖胺聚糖（GAG）侧链。以前，我们的实验室解决了比库宁PG的硫酸软骨素链的结构。当前的研究检查了decorin PG的硫酸皮肤素GAG链的复杂得多的结构。通过利用复杂的分离方法，然后进行成分分析，域映射和串联质谱分析，再结合改进的遗传算法方法进行的分析，确定了玉米芯蛋白硫酸皮肤素链的结构基序。这代表了具有突出的结构基序的GAG的第二个例子，表明这类糖缀合物的结构变异性比预期的要简单一些。
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