Use of azidonaphthalimide carboxylic acids as fluorescent templates with a built-in photoreactive group and a flexible linker simplifies protein labeling studies: applications in selective tagging of HCAII and penicillin binding proteins,Chemical Communications

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Use of azidonaphthalimide carboxylic acids as fluorescent templates with a built-in photoreactive group and a flexible linker simplifies protein labeling studies: applications in selective tagging of HCAII and penicillin binding proteins
Chemical Communications ( IF 4.9 ) Pub Date : 2017-11-06 00:00:00 , DOI: 10.1039/c7cc08209f
Monisha Singha _{1,

2,

3} , Sayantani Roy _{2,

3,

4} , Satya Deo Pandey _{2,

3,

5} , Subhendu Sekhar Bag _{1,

3,

6} , Prabuddha Bhattacharya _{1,

2,

3} , Mainak Das _{1,

2,

3} , Anindya S. Ghosh _{2,

3,

5} , Debashis Ray _{1,

2,

3} , Amit Basak _{1,

2,

3}

Affiliation

This work describes the synthesis of azidonaphthalimide carboxylic acids which act as fluorescent templates with a built-in photoreactive group and a linker thus simplifying the design of protein labeling agents. These were separately connected to selectivity hands like a sulfonamide and ampicillin for successful labeling/detection of HCAII and PBPs, respectively.

中文翻译：

使用叠氮邻苯二甲酰亚胺羧酸作为具有内置光反应性基团和柔性接头的荧光模板，可简化蛋白质标记研究：在HCAII和青霉素结合蛋白的选择性标记中的应用

这项工作描述了叠氮基邻苯二甲酰亚胺羧酸的合成，这些羧酸具有内置的光反应性基团和连接基，可作为荧光模板，从而简化了蛋白质标记剂的设计。将它们分别连接至选择性手，如磺酰胺和氨苄青霉素，分别用于成功标记/检测HCAII和PBP。

更新日期：2017-11-16

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