The fluorescent organic nanoparticles (FONs) with aggregation-induced emission (AIE) feature have recently attracted the most research interest owing to their well designability, outstanding optical properties and great potential for biomedical applications. Therefore, the development of novel strategies that could facilely fabricate AIE-active functional materials with high efficiency should be of great importance for their biomedical applications. In this work, a novel strategy that based on the microwave-assisted multicomponent tandem polymerization (MCP) has been developed to fabricate the biodegradable FONs with AIE feature. The AIE-active dye (NH₂-PhE-NH₂) with two amino groups, hydrophilic dialdehyde-terminating copolymer (PEG-PCL-CHO) and diethyl phosphite were successfully conjugated via a “one-pot” microwave-assisted Kabachnik–Fields (KF) reaction under rather mild conditions (e.g. air atmosphere, catalysts-free and solvent-free) within short reaction time (5 min). The obtained PEG-PCL-PhE FONs were characterized by a series of techniques. Results demonstrated that PEG-PCL-PhE FONs possess great water dispersibility, excellent optical properties as well as low cytotoxicity. Taken together, we developed a novel MCP strategy that based on the microwave-assisted KF reaction to fabricate AIE-active FONs with great physicochemical and biological properties. These AIE-active FONs are of great potential for biological imaging applications. Moreover, this strategy could also be adopted to fabricate many other AIE-active functional materials owing to its simple operation, high efficiency, short reaction time and substrate diversity.

具有聚集诱导发射（AIE）功能的荧光有机纳米粒子（FON）最近因其良好的可设计性，出色的光学性能以及在生物医学应用中的巨大潜力而​​引起了人们的最大研究兴趣。因此，开发可以方便地高效制造AIE活性功能材料的新策略对于其生物医学应用应该具有重要意义。在这项工作中，已开发出一种基于微波辅助多组分串联聚合（MCP）的新颖策略，以制备具有AIE功能的可生物降解FON。AIE活性染料（NH ₂ -PhE-NH ₂）具有两个氨基的亲水性二醛终止共聚物（PEG-PCL-CHO）和亚磷酸二乙酯通过“一锅法”微波辅助的Kabachnik-Fields（KF）反应在相当温和的条件下（例如，空气，在短的反应时间（5分钟）内不含催化剂和无溶剂）。通过一系列技术表征获得的PEG-PCL-PhE FON。结果表明，PEG-PCL-PhE FON具有良好的水分散性，优异的光学性能以及低细胞毒性。综上所述，我们开发了一种新颖的MCP策略，该策略基于微波辅助的KF反应来制备具有出色的理化和生物学特性的AIE活性FON。这些具有AIE活性的FON在生物成像应用中具有巨大的潜力。而且，