Uptake of Long Protein-Polyelectrolyte Nanotubes by Dendritic Cells,Biomacromolecules

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Uptake of Long Protein-Polyelectrolyte Nanotubes by Dendritic Cells
Biomacromolecules ( IF 5.5 ) Pub Date : 2017-11-13 00:00:00 , DOI: 10.1021/acs.biomac.7b01353
Diana G. Ramírez-Wong ₁ , Saghi Saghazadeh ₁ , Simon Van Herck ₂ , Bruno G. De Geest ₂ , Alain M. Jonas ₁ , Sophie Demoustier-Champagne ₁

Affiliation

Anisotropic nanostructures, such as nanotubes, incorporating bioactive molecules present interesting features for application as drug delivery carriers. Here, we present the synthesis of layer-by-layer (LbL) nanotubes including protein (ovalbumin) layers and go from simple to more complex synergetic combinations of synthetic and natural polyelectrolytes, leading to structures with tunable properties. The rigidity in organic and aqueous media, the stability in buffer solution and the uptake of different LbL tubes by dendritic cells (DCs) are analyzed to contrast size and chemistry. The most rigid studied systems appear as the best candidates to be internalized by cells, regardless of the chemistry of their outermost layers. The successful transport of long protein-loaded robust rigid nanotubes to the cytoplasm of DCs paves the way for their use as new cargo for the delivery of large amounts of antigen to such cells.

中文翻译：

树突状细胞对长蛋白-聚电解质纳米管的吸收

掺入生物活性分子的各向异性纳米结构，例如纳米管，呈现出有趣的特征，可以用作药物传递载体。在这里，我们介绍了包括蛋白质（卵清蛋白）层的逐层（LbL）纳米管的合成，并且从合成的和天然的聚电解质的简单到更复杂的协同组合进行合成，从而导致结构具有可调的特性。分析了树突状细胞（DC）在有机和水性介质中的刚性，在缓冲溶液中的稳定性以及不同LbL管的摄取，以对比大小和化学性质。研究最严格的系统似乎是细胞内部化的最佳候选者，无论其最外层的化学性质如何。

更新日期：2017-11-13

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