A Multivalent Structure-Specific RNA Binder with Extremely Stable Target Binding but Reduced Interaction to Nonspecific RNAs,Angewandte Chemie International Edition

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A Multivalent Structure-Specific RNA Binder with Extremely Stable Target Binding but Reduced Interaction to Nonspecific RNAs
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2017-11-13 04:46:34 , DOI: 10.1002/anie.201709153
Jeong Min Lee ₁ , Ahreum Hwang _{1,

2} , Hyeongjoo Choi ₁ , Yongsang Jo ₁ , Bongsoo Kim ₁ , Taejoon Kang _{2,

3} , Yongwon Jung ₁

Affiliation

Multivalent probe for a biosensor: An RNA binding monomer and multivalent scaffold proteins were optimized to develop a multivalent probe with near irreversible binding to target RNA and negligible binding to nonspecific RNA. The resulting ultra-specific 24-meric RNA binder allowed SERS detection of intact miRNAs at an attomolar level.

中文翻译：

具有非常稳定的目标结合，但减少了与非特异性RNA的相互作用的多价结构特异性RNA结合剂

用于生物传感器的多价探针：优化了RNA结合单体和多价支架蛋白，以开发出一种多价探针，其与靶RNA几乎不可逆地结合，而与非特异性RNA的结合可忽略不计。所产生的超特异性24聚体RNA结合物允许在大摩尔水平上对完整的miRNA进行SERS检测。

更新日期：2017-11-13

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