Preparation of hybrid-monomer, double-template molecularly imprinted polymers for the purification of green tea extracts,Analytical Methods

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Preparation of hybrid-monomer, double-template molecularly imprinted polymers for the purification of green tea extracts
Analytical Methods ( IF 2.7 ) Pub Date : 2017-10-30 00:00:00 , DOI: 10.1039/c7ay01842h
Qi Qu _{1,

2,

3,

4,

5} , Tao Zhu _{1,

2,

3,

4,

5}

Affiliation

Hybrid-monomer double-template molecularly imprinted polymers (HDMIPs) were prepared by two different approaches based on in situ polymerization. The hybrid-monomers were prepared from 3-aminopropyltriethoxysilane (APTES) (the inorganic precursor) and methacrylic acid (MAA)/acrylamide (AM) (the organic precursor). HDMIPs were used as sorbents for the selective isolation of theophylline and quercetin from green tea extracts. Fourier transform infrared and field emission scanning electron microscopy were used to characterize the morphology and structure of the HDMIPs. The recognition ability and binding capability of the HDMIPs for selected analytes were evaluated by static absorption and Scatchard analysis. The adsorption equilibria of the two analytes were investigated using three different analysis models: linear, Langmuir and Freundlich. With optimization of the solid-phase extraction procedure, a reliable analytical method was developed for recognition of theophylline and quercetin in green tea, with outstanding extraction recoveries of 96.68% and 88.92%, respectively. Using the proposed method, the recoveries at three spiked levels of analysis were in the range 84.62–96.68% for theophylline and 82.88–88.92% for quercetin. The HDMIPs therefore demonstrated a high affinity for the two target analytes, suggesting that the proposed approach could potentially offer a novel purification method for complex samples.

中文翻译：

制备混合单体，双模板分子印迹聚合物以纯化绿茶提取物

基于原位的两种不同方法制备了杂化单体双模板分子印迹聚合物（HDMIP）聚合。由3-氨基丙基三乙氧基硅烷（APTES）（无机前体）和甲基丙烯酸（MAA）/丙烯酰胺（AM）（有机前体）制备杂化单体。HDMIP被用作吸附剂，用于从绿茶提取物中选择性分离茶碱和槲皮素。傅里叶变换红外和场发射扫描电子显微镜被用来表征HDMIPs的形态和结构。通过静态吸收和Scatchard分析评估了HDMIP对选定分析物的识别能力和结合能力。使用三种不同的分析模型研究了两种分析物的吸附平衡：线性，Langmuir和Freundlich。通过优化固相萃取程序，开发了一种可靠的分析方法，用于识别绿茶中的茶碱和槲皮素，提取率分别为96.68％和88.92％。使用所提出的方法，茶碱的三个加标分析水平的回收率分别为84.62-96.68％和槲皮素的82.88-88.92％。因此，HDMIP对两种目标分析物表现出很高的亲和力，这表明所提出的方法可能为复杂样品提供一种新颖的纯化方法。

更新日期：2017-11-13

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