Facile and sensitive detection of clenbuterol in pork using a personal glucose meter,Analytical Methods

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Facile and sensitive detection of clenbuterol in pork using a personal glucose meter
Analytical Methods ( IF 2.7 ) Pub Date : 2017-10-23 00:00:00 , DOI: 10.1039/c7ay01826f
Fang Li _{1,

2,

3,

4,

5} , Ruihua Zhang _{1,

2,

3,

4} , Huaibin Kang _{1,

2,

3,

4,

5} , Yonggang Hu _{4,

6,

7,

8} , Yunhong Liu _{1,

2,

3,

4,

5} , Junya Zhu _{1,

2,

3,

4}

Affiliation

Clenbuterol (CLB) is a β-agonist drug that is illegally added as a growth-promoting agent in livestock. In this study, a facile and sensitive method for the detection of CLB using a personal glucose meter (PGM) was reported. Clenbuterol–bovine serum albumin functionalized magnetic beads were used to compete with CLB for binding to its corresponding antibody and gold nanoparticle–invertase–secondary antibody conjugates. In the sucrose solution, the invertase catalyzes the hydrolysis of sucrose to produce a large amount of glucose and quantitative readout using the PGM. The ratio of signals from the PGM before and after addition of CLB exhibits a good linear correlation with the logarithm of the CLB concentrations in the range of 0.1–100 ng mL⁻¹ with a detection limit of 0.1 ng mL⁻¹ (S/N = 3). Finally, the proposed method was successfully applied for the detection of CLB residues in pork and liver samples.

中文翻译：

使用个人血糖仪轻松灵敏地检测猪肉中的盐酸克仑特罗

克仑特罗（CLB）是一种β激动剂药物，被非法添加为家畜中的生长促进剂。在这项研究中，报告了一种使用个人血糖仪（PGM）检测CLB的简便而灵敏的方法。盐酸克仑特罗-牛血清白蛋白功能化磁珠用于与CLB竞争结合其相应的抗体和金纳米粒子-转化酶-第二抗体偶联物。在蔗糖溶液中，转化酶催化蔗糖水解以产生大量葡萄糖，并使用PGM定量读取。在添加CLB之前和之后来自PGM的信号比率与CLB浓度在0.1–100 ng mL ^-1范围内的对数具有良好的线性相关性，检出限为0.1 ng mL ^-1（S / N = 3）。最终，该方法成功地用于猪肉和肝脏样品中CLB残留的检测。

更新日期：2017-11-13

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